中文摘要：

将4-羟基-2，2，6，6-四甲基哌啶氮氧自由基用于标记9-羧基-吖啶，得到自旋标记荧光探针4-（9-吖啶酯）-2，2，6，6-四甲基哌啶氮氧自由基．以谷胱甘肽作为蛋白质肽模型，研究了活性氧过氧亚硝酸诱导其损伤产生的硫中心自由基的荧光表征．自旋标记荧光探针为弱荧光物质，当与硫中心自由基作用后，导致其荧光增强，从而可对硫中心自由基进行表征．

英文摘要：

A spin-labeling fluorophore（Ac-Tempo ）, formed by combining with an acridine with a paramagnetic nitroxide （4-hydroxyl-2,2,6,6-tetramethylpiperidinyl-1 -oxy）, was explored for peptide-based thiyl radicals generated through the reaction between gluththione and peroxynitrite. Ac-Tempo＇ , a weak fluorescent compound, showed a dramatic increase in fluorescence intensity after interaction with thiyl radicals, and could be employed to probe peptide-based thiyl radicals.