中文摘要：

目的 初步探讨仅通过观察行为学改变对锰中毒大鼠模型的判别作用。方法 将24只SPF级雄性SD大鼠按体重随机分为4组,每组6只,分别为对照组、低剂量组（15.0mg/kg）、中剂量组（25.0mg/kg）和高剂量组（50.0mg/kg）,染锰3个月,并对其进行行为学评分、肌张力改变、平衡木试验、水迷宫实验几项行为学测试,染锰结束后检测其大脑纹状体酪氨酸羟化酶（Tyrosine Hydroxylase,TH）表达含量,并以对照组大鼠平均水平的30.00%为界划分锰中毒组和非锰中毒组,通过多项行为学指标联合建立判别分析函数。结果 锰中毒组大鼠行为学评分、肌张力评分、平衡木评分均高于非锰中毒组（P〈0.01）,TH表达量锰中毒组低于非锰中毒组（P〈0.01）;Fisher判别法形成判别函数,符合率为91.67%（22/24）,误判率为8.33%（2/24）;该判别函数对非锰中毒组和锰中毒组的正确判别符合率均高于85.71%。结论通过行为学评分、肌张力改变、平衡木试验几项行为学测试组合对锰中毒性大鼠模型做判断初步可行。

英文摘要：

Objective To explore the discriminative effects about the behavioral changes of manganism rat. Methods Twenty four healthy male SD rats were randomly divided into the control group, the low dose group （15.0 mg/kg）, the middle dose group （25.0 mg/kg） and the high dose group （50.0 mg/kg）. Rats were subjected to behavioral test, muscle tension change,balance wood test and water maze test after intraperitoneal injection of manganese for three months, then the expression of Tyrosine Hydroxylase （TH） was detected in the striatum of the brain. All rats were divided into two groups by 30.000% of TH＇s expression., manganism and non-manganism. Then discriminant analysis functions were established according to the behavioral indicators above. Results The scores of the behavioral test,muscle tension change, balance wood test in manganism group were higher than those of the nonmanganism group（ P 〈0.01）. TH in the manganism group was lower than that in the non-manganism group（ P 〈0.01）. Fisher discriminant method to form the discriminant function,the coincidence rate was 91.67 % （22/24）, the error rate was 8.33 % （2/24）. The discriminant function of the two groups of correct discrimination coincidence rate was higher than 85.71%. Conclusion The manganism model rats can be judged by the behavioral changes,such as behavioral test,muscle tension change, snd balance wood test.