中文摘要：

利用改造后的溶瘤腺病毒Ad.enAFP-E1A-△E1B55Kd作为载体携带治疗基因Mn-SOD,得到目的病毒Ad.enAFP-E1A-△E1B55Kd-MnSOD;通过PCR方法鉴定病毒构建正确;MTT法检测病毒对肝癌细胞的特异杀伤能力和对肝正常细胞的安全性;Hoechst33342染色实验观察细胞凋亡的现象。结果显示：经目的病毒感染后肝癌细胞出现明显的病变现象和生长抑制,而肝正常细胞基本无病变现象出现,表明构建的目的病毒具有较高的安全性和很好的靶向性。此外目的病毒处理能引起特异性的肝癌细胞凋亡,所携带治疗基因Mn-SOD能通过促进肿瘤细胞的凋亡达到抑制肿瘤细胞生长的效果。

英文摘要：

In this study,the therapeutic virus Ad.enAFP-E1A-△E1B55Kd-MnSOD is constructed by the modified oncolytic adenovirus Ad.enAFP-E1A-△E1B55Kd as vectors to carry therapeutic genes Mn-SOD.Subsequently,the identification of correct virus construction by PCR,cell morphology changes and cell apoptosis after infected the tumor-specific killing effect and the evaluation of live normal cell security by MTT assay,and the detection of apoptosis by Hoechst assay are performed.The results indicate the obvious pathological changes and growth inhibition of hepatoma cells after infection of therapeutic viruses,while little impact on the liver normal cells with high security.Moreover,the treatment of therapeutic virus Ad.enAFP-E1A-△E1B55Kd-MnSOD significantly induces apoptosis of hepatoma cells through carrying the Mn-SOD gene,indicating the good targeting ability and the effect of inhibition of tumor cell growth.