中文摘要：

基于聚多巴胺纳米粒子（PDA NPs）对Cy5标记单链DNA（Cy5-ssDNA）探针的荧光猝灭效应以及脱氧核糖核酸酶Ⅰ（DNaseⅠ）选择性切割DNA/RNA杂合结构中单链DNA的特性,建立了一种用于微小核糖核酸（miRNA）检测的新型恒温信号放大方法.在优化的实验条件下,体系的相对荧光强度（FR）与miR-21浓度的对数值成正比;对miR-21检测的线性范围为10 pmol/L~100 nmol/L,检出限达7 pmol/L.血清加标实验结果表明,该方法可用于生理环境下miR-21的检测.

英文摘要：

A simple, rapid and low-cost polydopamine nanoparticle （PDA NP）-based fluorescence resonance energy transfer（FRET） assay with deoxyribonuclease I （DNase I ）-assisted target recycling signal amplifica- tion was developed for microRNA （miRNA） detection. Under the optimized experimental conditions, the recovery ratio of Cy5 fluorescence intensity increased linearly with logarithm of miR-21 concentration from 10 pmol/L to 100 nmol/L, with a detection limit of 7 pmol/L. Moreover, satisfactory results were obtained while the proposed method was applied to detect miRNA in 10% human serum.