中文摘要：

瞄准：构造实时稀释沙门氏菌 typhimurium (S。typhimurium ) 怀有 H pylori 的紧张嗜中性的激活蛋白质(HP 小睡) 基因作为一支口头的 recombinant DNA 疫苗，并且到评估它的 immunogenicity。方法：由遗传工程方法， H pylori 的 genomic DNA 作为一个模板被提取。HP 小睡基因的全部的长度被聚合酶链反应(PCR ) 放大并且为定序和强风分析克隆向量进 pBT，然后，克隆进真核细胞的表示向量 pIRES 的潜水艇由 PCR 鉴定和限制酶消化列在后面。识别 recombinant 原生质标志 pIRES 小睡是进为目标熔化蛋白质表示的 COS-7 房间的 transfected，并且它的抗原性被西方的弄污检测。然后， recombinant 原生质标志被转变成实时稀释 S。typhimurium 拉紧一支口头的疫苗拉紧的 SL7207，并且它的 immunogenicity 与动物实验被评估。结果：435 bp 产品在 GenBank (超过 98%) 与 HP 小睡基因用高相同被克隆。与由 PCR 和限制酶消化的鉴定， recombinant 包含 H pylori 的 HP 小睡基因的真核细胞的表示原生质标志 pIRES 小睡成功地被构造。表示目标蛋白质与 H pylorii 全部房间抗体有特异性反应并且显示出西方的弄污检测的单个长带结果。有 recombinant DNA 疫苗的紧张 SL7207 (pIRES 小睡) 的老鼠的口头的免疫也导致了特定的有免疫力的回答。结论：与好 immunogenicity 疫苗的 HP 小睡口头的 DNA 的成功的构造可以帮助进一步对 H pylori 感染调查它的免疫保护效果和 develop 疫苗。

英文摘要：

AIM： To construct a live attenuated Salmonella typhimurium （S. typhimurium） strain harboring the H pylori neutrophil activating protein （HP-NAP） gene as an oral recombinant DNA vaccine, and to evaluate its immunogenicity. METHODS： By genetic engineering methods, the genomic DNA of Hpylori was extracted as a template. The total length of the HP-NAP gene was amplified by polymerase chain reaction （PCR） and cloned into pBT vector for sequencing and BLAST analysis, then subcloned into a eukaryotic expression vector pIRES followed by PCR identification and restriction enzyme digestion. The identified recombinant plasmid pIRES-NAP was transfected into COS-7 cells for target fusion protein expression, and its antigenicity was detected by Western blotting. Then the recombinant plasmid was transformed into a live attenuated S. typhimurium strain SL7207 as an oral vaccine strain, and its immunogenicity was evaluated with animal experiments. RESULTS： A 435 bp product was cloned using high homology with HP-NAP gene in GenBank （more than 98%）. With identification by PCR and restriction enzyme digestion, a recomoinant eukaryotic expression plasmid pIRES-NAP containing the HP-NAP gene of H pylori was successfully constructed. The expressed target protein had a specific reaction with Hpyloril whole cell antibody and showed a single strip result detected by Western blotting. Oral immunization of mice with recombinant DNA vaccine strain SL7207 （pIRES-NAP） also induced a specific immune response. CONCLUSION： The successful construction of HP-NAP oral DNA vaccine with good immunogenicity may help to further investigate its immunoprotection effects and develop vaccine against Hpylori infection.