中文摘要：

目的 探讨SH-SY5Y细胞经锰染毒后Hsp10表达与细胞活力、凋亡率之间的关系. 方法 将受试SH-SY5Y细胞分为对照组和实验组,A组（0.9％ NaCl）作为对照组,B组（Mn2＋0.5 mmol/L）、C组（Mn2＋ 1.0 mmol/L）、D组（Mn2＋ 2.0mmol/L）和E组（Mn2＋ 4.0 mmol/L）为实验组.观察锰不同剂量染毒12 h后SH-SY5Y细胞活力、凋亡率的变化;实时荧光定量PCR（RT-PCR）检测Hsp10 mRNA的相对表达水平,Western blot检测Hsp10蛋白表达情况. 结果 细胞染毒以后,与对照组比较,不同浓度Mn2组的细胞活力显著下降（P＜0.05）,分别为对照组的92.93％,87.28％,79.53％和73.67％,与对照组相比差异有统计学意义（P＜0.05）;各浓度Mn2＋组凋亡率呈明显的剂量反应关系,随Mn2＋染毒浓度增加而上升;Hsp10基因的表达水平（mRNA和蛋白）也呈显著的剂量反应关系,各浓度组Hsp10 mRNA的相对表达分别是对照组的0.95,0.84,0.63,0.46倍,各浓度组Hsp10蛋白含量与A组相比差异有统计学意义（P＜0.05）. 结论 Mn可引起SH-SY5Y细胞活力逐渐下降,凋亡率逐渐上升,Hsp10表达水平（mRNA和蛋白）下降,提示Hsp10可能在Mn诱导细胞凋亡过程中发挥重要的作用.

英文摘要：

Objective To explore the relationship between cell viability,apoptosis rate and heat shock protein 10 （Hsp10） expression after SH-SY5Y cells exposure to manganese（Mn2＋）.Methods SH-SY5Y cells were divided into control group （group A,normal saline,0.9％ NaCl） and experimental groups （group B：Mn2 ＋ 0.5 mmol/L,group C：Mn2 ＋ 1.0 mmol/L,group D：Mn2 ＋ 2.0 mmol/L,group E：Mn2＋ 4.0 mmol/L）.After SH-SY5Y cells exposed to Mn2＋ for 12 h,the cell viability and apoptosis rate in each group was measured by MTT method,and expression of Hsp10 mRNA and protein was detected by RT-PCR and Western blot.Results Compared to control group,the cell viability significantly decreased in each experimental group （P ＜ 0.05）,which was 92.93％,87.28％,79.53％ and 73.67％,respectively.The apoptosis rate increased in a dose-dependent manner after exposure to Mn2＋.Similarly,the mRNA and protein expression of Hsp10 presented a notably dose-dependent decrease.Hsp10 mRNA level was 0.95,0.84,0.63,0.46 folds of the control in groups B,C,D,E and the Hsp10 protein expression was significantly lower than in control group（P ＜0.01）.Conclusion Manganese can attenuate the cell viability and Hsp10 expression （mRNA and protein） but elevate the rate of apoptosis,which suggest that Hsp10 might play an important role in Mn-induced apoptosis.