中文摘要：

目的 探讨银莱汤对食积复合流感病毒感染小鼠肠黏膜免疫屏障的作用机制,论证肺与胃肠同治理论的有效性,为食积呼吸道感染疾病的防治提供实验依据.方法 小鼠随机分为正常组、感染组、食积组、食积感染组、食积治疗组、食积感染治疗组.实验周期8天.正常组、感染组喂饲普通饲料,其余各组于实验第1天至第4天喂饲高蛋白高热量饲料,并以牛奶溶液灌胃.感染组、食积感染组于实验第4天滴鼻感染流感病毒.食积治疗组、食积感染治疗组于实验第4天至第8天给予银莱汤治疗.取材观察实验各组肠黏膜分泌型免疫球蛋白A （sIgA）、肿瘤坏死因子α（TNF-α）、白细胞介素10（IL-10）水平.结果 食积组与食积感染组小鼠的肠黏膜sIgA、TNF-α、IL-10水平较正常组显著下降（P＜0.01）；食积治疗组sIgA、TNF-α、IL-10水平显著高于食积组（P＜0.01）,其中TNF-α水平显著低于正常组（P＜0.01）,sIgA、IL-10水平与正常组比较无显著性差异（P＞0.05）；食积感染治疗组sIgA、TNF-α、IL-10水平显著高于食积感染组及正常组（P＜0.01）.结论 银莱汤能够增加肠黏膜分泌sIgA,适度调节细胞因子TNF-α、IL-10水平,对肠黏膜机械屏障和免疫屏障具有保护作用.

英文摘要：

Objective To discuss the therapeutic mechanism of Yinlai Tang to mucosa immunologic barrier of mice with dyspepsia combined with influenza virus infection, to demonstrate the effectiveness of treating lung and stomach simultaneously, and to provide experimental evidence for dyspepsia combined with respiratory tract infection prevention and treatment. Methods Mice were randomly divided into normal group, infection group, dyspepsia group, dyspepsia and infection group, dyspepsia treated group and dyspepsia and infection treated group. The experimental period was 8 days. The mice in normal group and infection group were fed with standard diet, and the mice in the other groups were fed with high fat and high calorie diet and given intragastric administration of milk from the 1 st day of experiment to the 4th day. The mice in infection group and dyspepsia and infection group were given intranasal instillation of influenza virus at the 4th day. The mice in dyspepsia treated group and dyspepsia and infection treated group were given Yinlai Tang from the 4th day to the 8th day. The levels of secretedimmunoglobulin A （sIgA）, tumor necrosis factor-a mucosa of mice of all groups were detected. Results （TNF-α） and interleukin-10 （IL-10） in intestinal The levels of sIgA, TNF-α and IL-10 in intestinal mucosal tissues of mice decreased significantly in dyspepsia group as well as dyspepsia and infection group compared with those in normal group （ P 〈 0. 01 ）. The levels of sIgA, TNF-α and IL-10 in dyspepsia treated group were significantly higher than those in dyspepsia group （ P 〈 0.01 ）, and the level of TNF-α in dyspepsia treated group was significantly lower than that in normal group （ P 〈 0. 01 ）, but there was no significant difference in sIgA and IL-10 between dyspepsia treated group and normal group （P 〉 0. 05 ）. The levels of sIgA, TNF-α and IL-IO in dyspepsia and infection treated group were significantly higher than those in dyspepsia and infection group and normal group （P