中文摘要：

为研究海南普通野生稻中的STK类抗病基因,根据已知植物抗病基因NBS（Nucleotide binding site）序列中的保守区域设计简并引物,以海南普通野生稻基因组DNA为模板扩增得到4条具有连续ORF的抗病基因类似物（Resistance gene analogues,RGAs）序列,它们核苷酸序列间的相似性系数在53.14%-99.81%之间,而相应推测的氨基酸序列间的相似性系数在39.08%-99.42%之间。对氨基酸序列进行结构分析表明,它们包括＂P-loop＂＂、Kinase-2a＂＂、Kinase-3a＂和＂GLPL＂4个抗病基因所共有的保守模体,并且4条海南普通野生稻NBS-LRR类似物均属于nonTIR-NBS类抗病基因片段。

英文摘要：

For study on STK resistance genes in Hainan common wild rice, degenerate primers based on conserved motif of the nucleotide binding site （NBS） region from the cloned plant disease resistance genes were used to isolate resistance gene analogues （RGAs） from genomic DNA of Hainan common wild rice （Oryza rufipogon Griff） and 4 RGAs with uninterrupted open reading frames （ORFs） were obtained. Sequence identity among the 4 RGAs nucleotide sequences ranged from 53.14% to 99.81%, while the 4 RGAs deduced amino acid sequences showed identity ranged from 39.08% to 99.42%. The deduced amino acid sequences of RGAs contain the conserved domains such as P-loop, Kinase-2, Kinase-3a and GLPL, and the 4 RGAs all belonged to nonTIR-NBS type resistance gene segments.