中文摘要：

目的观察吉马酮对人肺鳞癌LK2细胞的抑制作用，并对其作用机制进行探讨。方法体外培养LK2细胞，采用MTT法观察吉马酮对LK2细胞增殖的影响，采用吖啶橙染色法观察吉马酮作用前后细胞形态学上的差异及凋亡程度，采用流式细胞术检测细胞凋亡率变化。结果吉马酮对LK2细胞体外增殖有抑制作用，且呈明显的剂量依赖性，差异有统计学意义（P〈0．05）；吖啶橙染色结果表明：吉马酮处理细胞可见核碎裂等典型的凋亡改变；流式细胞仪检测结果显示：LK2细胞凋亡率随吉马酮作用浓度增加而增加，呈剂量依赖性。结论吉马酮可以抑制人肺鳞癌LK2细胞的增殖并诱导细胞凋亡。

英文摘要：

Objective To study the influence of germacrone on the proliferation and apoptosis in human squamous cell lung carcinoma LK2 cells. Methods Human squarnous cell lung carcinoma LK2 cells were cultured in vitro. The growth inhibitory effect of germacmne on LK2 cells was detected by MTT assay. The morphology of the apoptotic cells was examined by acridine orange staining. Apoptosis rate was determined by flow cytometry analysis using an Annexin V-FITC Apoptosis Kit. Results The growth of LK2 cells was inhibited by germa- crone in a dose-dependem manner （P 〈 0.05）. Nuclear fragmentations were found clearly in germacrone-treated cells by acridine orange staining. Flow cytometric analysis showed that germacrone could induce LK2 cells apoptosis in a dose-dependent manner. Conclusion Ger- macrone could inhibit human squamous cell lung carcinoma LK2 cells proliferation and induce cell apoptosis.