采用平板变色法,从23种白腐菌菌株中定性筛选出18株白腐菌。参与复筛的18株白腐菌降解黑杨30d后,以Klasson木质素降解率与综纤维降解率的比值作为筛选依据,最终发现3株选择性脱木质化的菌株:白赭多年卧孔菌Perenniporia ochroleuca D9597、粗毛纤孔菌Funalia trogii C6978和东方栓孔菌Trametes orientalis C6320。此外通过主成分分析降解机制,上述参与复筛的18株白腐菌菌株在降解黑杨30d的历程中存在3种降解类型:A类型的菌株能选择性降解黑杨木质素,B、C类型的菌株都具有选择性降解黑杨综纤维素的能力,B、C类型的菌株针对综纤维素的降解非常强烈。另外,筛选出的菌株P.ochroleuca D9597、F.trogii C6978及T.orientalis C6320分解黑杨木粉20d后,在紫外扫描分析木粉的乙醇提取液时发现,其中的木质素发生了显著的分解作用。
Based on agar plate tests, 18 isolates from 23 white rot fungi were selected to a subsequent wood block decay test. According to the ratio of Klasson lignin losses to holocellulose losses, three isolates, Perenniporia ochroleuca D9597, Funalia trogii C6978 and Trametes orientalis C6320, showed selective delignification on Populus nigra. Based on primary component analysis, three different types of degradation were found during the fermentation of P. nigra by 18 white rot fungi: type A represents selective delignification; types B and C can selectively decompose holocelluloses, but type C has a stronger capacity than type B. Investigation was carried out on the UV absorbance spectra of ethanol extracts of wood particles treated by P. ochroleuca D9597, F. trogii 20 days, and lignin in the treated wood was found to be decomposed C6978 and T. orientalis C6320 for markedly