中文摘要：

目的 比较甲状腺相关性眼病（TAO）患者与健康人泪液中的溶菌酶C和乳铁蛋白的表达差异.方法 泪液样品采自TAO活跃期的患者25眼（病例组）和年龄、性别相匹配的健康志愿者25眼（对照组）.将泪液标本进行十二烷基硫酸钠-聚丙烯酰胺（SDS-PAGE）凝胶电泳,采用15％的凝胶分离两组样品的差异蛋白条带,收集标记的差异蛋白条带进行凝胶内胰蛋白酶消化,然后进行质谱分析,以确定在差异条带中的蛋白质组分.Scaffold搜索引擎分析质谱的结果,进行蛋白质的鉴定.结果 通过对差异蛋白条带的质谱分析,发现在TAO患者泪液中大多数蛋白表现为下调或者缺失,但是溶菌酶C和乳铁蛋白却发生了上调.鉴定蛋白的相对定量比值（病例/对照）：溶菌酶C为4.88,乳铁蛋白为1.60.结论 TAO患者泪液中的溶菌酶C和乳铁蛋白均发生上调,说明两者可能参与了TAO的炎症过程.

英文摘要：

Objective To explore the differential expressions of lysozyme C and lactoferrin in tears of thyroid-associated ophthalmopathy （TAO） patients versus healthy subjects by proteomics.Methods Tear samples were obtained from patients with active period TAO and age and gender-matched healthy subjects without symptoms of ocular surface.Then they were divided into patient and control groups.Then tear samples of two groups were analyzed.sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDSPAGE） of 15％ gel was performed to determine the different protein bands in sample groups.And different labeled protein bands were collected for in-gel tryptic digestion.Mass spectrometry was employed to determine the protein components from different protein bands.Then Scaffold search engine was used for analyzing the results of mass spectrometry and identifying specific proteins.Results Based on mass spectrometric analysis of different protein bands,most proteins were down-regulated or became absent in TAO patients.Both lysozyme C and lactoferrin were up-regulated.Identification of protein relative quantitative ratio （patient/control）：lysozyme C：4.88,lactoferrin：1.61.Conclusions Lysozyme C and lactoferrin are two important effectors of tear function and metabolism.Both are up-regulated in TAO patients＇ tears.Thus both are probably involved in inflammatory process of TAO and play synergistic roles in the pathogenesis of disease.