中文摘要：

目的：探讨柴苓小方对大鼠肾小球系膜细胞（GMCs）异常增殖的影响及相关机制。方法：采用脂多糖刺激系膜细胞异常增殖。MTT法检测柴苓小方对㈣异常增殖的影响；免疫细胞化学方法检测药物作用后增殖细胞核抗原（PCNA）的表达；乳酸脱氢酶释放实验检测柴苓小方的细胞毒作用。采用RT—PCR检测药物作用不同时间点细胞周期蛋白依赖性激酶抑制剂p21的表达。结果：柴苓小方可以剂量依赖性的抑制系膜细胞异常增殖并显著减少PCNA阳性细胞数而没有明显的细胞毒作用。并且柴苓小方可以显著地上调p21基因的表达（P〈0．05）。结论：柴苓小方可剂量依赖性的抑制GMC8异常增殖．其机制可能与上调p21mRNA表达有关。

英文摘要：

Objective： To investigate the effects and mechanism of Chai- Ling formula on the abnormal proliferation of glomerular mesangial cells（GMCs） in rats. Methods： GMCs abnormal proliferation were induced by lipopolysaccharide（LPS）. The influence of Chai - Ling formula on the abnormal proliferation of GMCs was determined by MTT assay and the expression of the proliferative cell nuclear antigen （PCNA） was determined by immunocytochemical detection; LDH release experiment was used to examine the cytotoxicity of the Chai- Ling formula. Reverse transcription polymerase chain reaction （RT- PCR） was used to examine the Cyclin- Kinase Inh/bitor（CKI） p21 mRNA at 3 h,6 h, 12 h, 24 h and 48 h after treated by Chai- Ling formula. Results：Chai -Ling formula inhibited the proliferation of GMCs in a concentration- dependent manner without obvious cytotoxicity（ P 〈 0.01 ）. Immunocytochemical study for PCNA showed the number of positive cells was significantly decreased in treated group compared with those in control group （P 〈 0.01 ）. Furthermore, Chai - Ling formula can significantly stimulated the expression of p21 mRNA （P〈 0.05）. Conclusion： Chai- Ling formula could inhibit the abnormal proliferation of GMCs in a concentration- dependent manner. The mechanism might be Chai - Ling formula upregulating the expression of the p21 mRNA.