中文摘要：

目的观察大鼠缺血再灌注损伤肾组织Trps1的表达变化及其与肾组织病变的关系,探讨Trps1在肾脏修复再生中的作用。方法 42只雄性SD大鼠分为假手术组、缺血再灌注组。经腹正中切口分离大鼠双侧肾蒂,用无损动脉夹夹闭60 min后恢复灌注,假手术组仅暴露肾蒂不夹闭。于再灌注后1、3、7、14、21 d取大鼠动脉血及肾组织标本,采用自动生化分析仪检测大鼠血肌酐、尿素氮水平,PAS染色检测肾组织损伤情况,免疫组化和Western blot检测肾组织Tprs1表达变化。结果假手术组大鼠血肌酐和尿素氮分别为（30.12±2.95）μmol/L、（5.89±0.67）mmol/L。与假手术组相比,大鼠肾脏缺血再灌注后1、3、7 d血肌酐[（319.25±25.49）、（338.20±22.70）、（216.07±18.54）μmol/L]及尿素氮[（33.53±8.06）、（78.05±7.13）、（42.61±11.87）mmol/L]显著升高（P〈0.05）,14、21 d血肌酐[（33.33±6.46）、（32.06±8.74）μmol/L]及尿素氮[（6.02±1.27）、（6.93±3.28）mmol/L]与假手术组相比无显著差异（P〉0.05）。PAS染色显示肾组织在再灌注1 d时即出现损伤,3 d时损伤最重,7 d时损伤开始修复,至第21天修复完全。免疫组化及Western blot显示,假手术组Trps1主要在皮质肾小管上皮细胞表达,缺血再灌注后1 d肾组织Trps1表达显著下降（P〈0.05）,3 d仅见微弱表达（P〈0.05）,7、14 d表达开始上升,但仍有显著差异（P〈0.05）,21 d与假手术组水平无显著差异（P〉0.05）。相关性分析显示Trps1表达变化与肾小管急性损伤评分呈负相关（r=-0.81,P〈0.05）。结论 Trps1在AKI肾脏损伤修复期表达上调,与肾组织损伤指标呈负相关,提示其可能具有促进肾脏修复再生的作用。

英文摘要：

Objective To study Trps1 expression changes in rat kidney after ischemia/reperfusion（I/R） injury and its relationship with renal pathological changes so as to explore the role of Trps1 in renal repair and regeneration.Methods Forty-two male SD rats were divided to a sham operation group and an I/R group.Kidney I/R rat model was established by clipping bilateral renal pedicles by artery clamps for 60 min after isolating them from surrounding tissues.On days 1,3,7,14 and 21 after kidney I/R,SCr and BUN were detected.Renal injury was observed under a light microscope with PAS staining,and Trps1 was tested by immunohistochemistry and Western blotting.Results The SCr and BUN levels in the sham operation group were（30.12±2.95） μmol/L and（5.89±0.67） mmol/L.The levels of SCr [（319.25±25.49）,（338.20±22.70）,and（216.07±18.54） μmol/L] and BUN [（33.53±8.06）,（78.05±7.13）,and（42.61±11.87） mmol/L] on days 1,3,and 7 were significantly higher in the I/R group than in the sham operation group（P〈0.05）.No significant difference was observed in SCr [（33.33±6.46） and（32.06±8.74） μmol/L] and BUN [（6.02±1.27） and（6.93±3.28） mmol/L] levels between the two groups on days 14 and 21（P〈0.05）.PAS staining results showed that kidney injury started on day 1 and was the severest on day 3,while kidney repair started on day 7 and completed on day 21.Immunohistochemistry results showed that Trps1 was mainly expressed in kidney tubules of cortex in the sham operation group.In the I/R group,Trps1 expression in kidney tissue decreased significantly on day 1 after I/R（P〈0.05）,was weak on day 3（P〈0.05）,started to increase on days 7 and 14 but still had significant difference（P〈0.05）,and returned to the same level as the sham operation group on day 21（P〈0.05）.The expression of Trps1 was negatively correlated with kidney tubule injury（P〈0.05）.Conclusion Trps1 shows upregulated expression during kidney repair after acute kidney injury,and is negative