中文摘要：

目的观察小檗碱（Berberine，BBR）对鼻咽癌细胞埃兹蛋白（Ezrin）和磷酸化埃兹蛋白（phos-Ezrin）表达的影响以及其对细胞伪足形成和迁移的抑制作用，探讨BBR抗鼻咽癌转移可能的分子机制。方法以体外培养的鼻咽癌细胞株（CNE1）为研究对象，用MTr法分析BBR对CNE1细胞生长的影响，选用BBR细胞无毒性浓度（non-cytotoxic concentration，NCC）进行实验。免疫印迹（Western-blotting）分析CNE1Ezrin和phos-Ezrin的表达。用光镜CNE1观察细胞迁移能力，电子显微镜观察细胞伪足形成。Ezrin小干扰RNA（Ezrin-siRNA）转染阻断Ezrin蛋白表达，进一步确证BBR通过Ezrin抑制细胞伪足形成。结果MTT结果显示，BBR在5μM以上明显抑制CNE1增殖（P〈0．05），浓度（0—5）州对CNE1细胞生长增殖抑制作用不明显，为BBR对CNE1的细胞无毒性浓度（NCC）。BBR在NCC能明显抑制CNE1细胞phos-Ezfin表达，且呈浓度依赖性。光镜观察显示BBR对CNE1细胞迁移有明显的抑制作用。电镜观察结果显示，BBR抑制CNE1细胞伪足形成。结论BBR可能通过抑制Ezfin磷酸化而抑制鼻咽癌CNE1迁移。

英文摘要：

Objective To observe the effect of Berberine on the expressions of Ezrin and phos- Ezrin and the motility of nasopharyngeal carcinoma （ NPC ） cell line （ CNE 1 ） , and to investigate the anti- metastatic mechanism of Berberine for NPC. Methods MTT assay was used for determining the inhibitory effect of Berberine on CNE 1. After treated with Berberine at a non-cytotoxic concentration （ NCC ） , we detected the expression of Ezrin and phos-Ezrin using Western Blot, and observed the motility and pseudopod under microscope, siRNA-Ezrin was transferred into CNE 1 , and then pseudopod of CNE 1 was observed. Results MTT assay data showed that BBR could inhibit the proliferation of CNE 1 at the concentration greater than 5 μM （ P 〈0. 05） , and no effect less than 5 μM. The NCC of BBR was 0 - 5 μM. BBR could inhibit the expression of phos- Ezrin, pseudopod formation and the motility of CNE 1 at NCC （ 0 - 5μM ）. Conclusion BBR may inhibit the metastasis of NPC via inhibiting phospharylation of Ezrin.