中文摘要：

应用气相质谱测定小鼠体内甲苯二异氰酸酯（TDI）的代谢产物,并鉴别了其在机体中的代谢途径。TDI色谱条件为Supelco PTETM-5色谱柱（30 mm×0.25 mm×0.25μm）,起始柱温90℃保持30 min,以40℃·min^-1升至280℃,保持5.25 min;汽化室温度250℃;载气为氦气,流速为1.0μL·min^-1。TDI体内代谢产物色谱条件为30＋2 mX0.25＋0.02 mm 94%甲基、4%乙烯基键合相弹性石英毛细柱,起始温度30℃保持5 min,以8℃·min^-1升至280℃,保持5 min。汽化室温度250℃;载气为氦气,流速1.0μL·min^-1。质谱条件为电离方式EI,电离能量70 eV,连接管温度280℃,扫描范围35-350μ;进样量1.0μL。结果表明2,4甲苯二异氰酸酯在血液、尿液、粪便中都被代谢成为2,4甲苯二胺。GC-MS法可有效分离并鉴定TDI体内代谢产物。

英文摘要：

In the present research we used gas chromatography-mass spectrometry（GC-MS）to determine metabolites of toluene diisocyanate（TDI） in mice and deduce the pathway for toluene diisocyanate metabolism in the organism.Conditions for TDI chromatography： Supelco PTETM-5 chromatographic column（30 mm×0.25 mm×0.25 μm）;initial column temperature： 90 ℃,which was maintained for 30 min,then the temperature was increased at a rate of 40 ℃·min^-1 to 280 ℃,and maintained for 5.25 min;temperature for the vaporizing chamber： 250 ℃;carrier gas： helium flowing at 1.0 μL·min-1.Conditions for chromatography of TDI metabolites in the organism： 94% methyl,4% ethenyl-bonded-phase fused-silica capillary column（30＋2 mX0.25 ＋ 0.02 mm）;initial column temperature： 30 ℃,which was maintained for 5 min,after and then was increased at a rate of 80 ℃·min^-1 to 280 ℃,and maintained for 5 min;temperature for the vaporizing chamber： 250 ℃;carrier gas： helium flowing at 1.0 μL·min^-1.Conditions for mass spectrometry： EI for ionizati on;70 eV for ionization energy;280 ℃ for connecting tube temperature;35-350 μ for range of scanning;and 1.0 μL for sample size.The results showed that 2,4-toluene diisocyanate was metabolized into 2,4-diaminotoluene.Under the conditions selected for GC-MS,TDI metabolites in the organism can be isolated and identified.