中文摘要：

目的将创造为理解 prion 蛋白质(PrP ) 的生理的功能和病理表示 hamster-PRNP 和 human-PRNP 为一个模型的转基因的老鼠，以及能递送的海绵状的 encephalopathies (TSE ) 的跨种类的传播的机制。方法仓鼠和 human-PRNP 转基因的老鼠被常规方法建立。在各种各样的老鼠线的综合 PRNP 的拷贝数字被即时 PCR 印射。PRNP mRNA 和蛋白质层次被半量的 RT-PCR，即时 RT-PCR，和西方的污点分析决定。转基因的老鼠的组织学的分析被染色的 hematoxylin 和曙红和 immunohistochemical (IHC )(H 与 E ) 执行方法。结果在各种各样的老鼠线的综合 PRNP 拷贝数字是 53 (Tg-haPrP1 ) ， 18 (Tg-huPrP1 ) ， 3 (Tg-huPrP2 ) ，并且 16 (Tg-huPrP5 ) 分别地。外长的 PrPs 在 transcriptional 和翻译水平被表示。组织学的试金没在大脑或另外的机关检测任何畸形。我们建立了一 hamster-PRNP 的结论转基因的老鼠线和三 human-PRNP 转基因的老鼠线。这四根转基因的老鼠线为另外的研究提供理想的模型。

英文摘要：

Objective To create transgenic mice expressing hamster- and human-PRNP as a model tor understanding the physiological function and pathology of prion protein （PrP）, as well as the mechanism of cross-species transmission of transmissible spongiform encephalopathies （TSEs）. Methods Hamster and human-PRNP transgenic mice were established by conventional methods. The copy number of integrated PRNP in various mouse lines was mapped by real-time PCR. PRNP mRNA and protein levels were determined by semi-quantitative RT-PCR, real-time RT-PCR, and western blot analysis. Histological analyses of transgenic mice were performed by hematoxylin and eosin （H ＆ E） staining and immunohistochemical （IHC） methods. Results Integrated PRNP copy number in various mouse lines was 53 （Tg-haPrP1）, 18 （Tg-huPrP1）, 3 （Tg-huPrP2）, and 16 （Tg-huPrP5）, respectively. Exogenous PrPs were expressed at both the transcriptional and translational level. Histological assays did not detect any abnormalities in brain or other organs. Conclusion We have established one hamster-PRNP transgenic mouse line and three human-PRNP transgenic mouse lines. These four transgenic mouse lines provide ideal models for additional research.