土壤厌氧培养、添加CH4氧化抑制剂和稳定性C同位素方法是3种主要的稻田CH4氧化研究方法。采用土壤厌氧培养方法估算的CH4氧化率偏高,其大部分研究结果中CH4氧化率均〉50%,高于其他方法的研究结果。添加CH4氧化抑制剂不仅会抑制CH4的氧化,同时也会减少或促进CH4的生成,这与添加的CH4氧化抑制剂的浓度有关。稳定性C同位素方法可在自然条件下测定CH4氧化率,无破坏性、灵敏度高,但计算CH4氧化率输入的δinitial、δfinal和a值还需要进一步研究。
Three methods have been mainly used to quantify methane oxidation in paddy fields, namely, soil anaerobic incubation, the use of methane oxidation inhibitors and stable carbon isotopes. Methane oxidation ratios measured by soil anaerobic incubation were overestimated, and most of the values obtained by this method were above 50%, higher than the values obtained by other methods. In the inhibition studies, methanogenesis was reduced or stimulated while methane oxidation was blocked, depending on the inhibitor's concentration. Stable carbon isotopes used to quantify methane oxidation was nondestructive and more sensitive than other methods, but further studies on δinitial, δfinal and a were still necessary.