中文摘要：

本文利用电解剥离法制得石墨烯/DNA复合材料,通过化学还原法将纳米金颗粒固定在石墨烯/DNA复合材料表面制得石墨烯/DNA/纳米金（ Gr/DNA/GNPs）复合材料,最终构建了一种基于Gr/DNA/GNPs修饰电极的无酶葡萄糖生物传感器。通过循环伏安法考察了不同修饰电极在碱性葡萄糖溶液中的电化学行为,并探讨了溶液中OH-离子强度、扫描电位范围及Gr/DNA/GNPs修饰量对传感器响应特性的影响。在优化实验条件下,采用循环伏安法检测葡萄糖的线性范围为8．0×10-5-5．0×10-2 mol·L-1,检出限为1．2×10-5 mol·L-1（S/N=3）。对2．0×10-3 mol·L-1的葡萄糖平行测定5次,其相对标准偏差为3．2%。实验结果表明该传感器具有较高的灵敏度、较好的重现性、稳定性及抗干扰能力。本方法可成功用于人血清样品中葡萄糖含量的测定,回收率为97．4%-102．8%。

英文摘要：

In this paper,graphene/DNA composites were synthesized by electrolytic exfoliation,and a chemical reduction method was used to fix the gold nanoparticles on the surface of graphene/DNA composites to produce the graphene/DNA/gold nanoparticles （ Gr/DNA/GNPs） composites. Finally,a novel non-enzymatic biosensor for glucose based on Gr/DNA/GNPs modified electrode was prepared. The electrochemical behaviors of glucose in alkaline solution on different modified electrodes were studied by cyclic volta-mmetry. Meanwhile,the effects of the intensity of OH- ions in solution,scanning potential range and the modified amount of Gr/DNA/GNPs on the sensor response characteristics were studied. Under optimal conditions, the glucose biosensor shows a linear range from 8. 0×10-5 to 5. 0×10-2 mol·L-1 with a detection limit of 1. 2×10-5 mol·L-1（S/N=3）. The relative standard deviation was 3. 2%for 2. 0×10-3 mol·L-1 glucose（n=5）. The biosensor shows high sensitivity,good reproducibility,stability and can avoid the commonly coexisted interference. The established method was successfully applied for the determination of glucose in human ser-um samples with satisfactory results,and the recovery was between 97. 4%and 102. 8%.