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中文摘要：
通过自由基共聚合方法成功制备了γ-聚谷氨酸温敏凝胶P（γ-PGA-co-NIPAAm）,研究发现r=0.05、0.10和PNIPAAm凝胶分别在33.1,33.6和32.5℃处发生可逆的温度相转变行为。与PNIPAAm凝胶相比,共聚凝胶更利于L929的细胞粘附和增殖,表明引入聚谷氨酸利于L929细胞的生长。通过控制温度变化,细胞从凝胶表面自动脱附,其活性大于PNIPAAm凝胶表面自动脱附和从TCPS板上通过酶解法得到的细胞的生物活性。
英文摘要：
A novel thermo-responsive and biocompatible copolymer hydrogel based on N-isopropylacrylamide （NIPAAm）and polyγ-glutamic-based macromonomer （γ-PGA-AGE）was synthesized successfully by the free radical copolymerization.The copolymer hydrogel with r=0.05,r=0.10 and PNIPAAm hydrogel exhibits re-versible temperature-response at about 33.1,33.6 and 32 ℃ in water.Compared with conventional PNIPAAm hydrogel,the proliferation of L929 cells were higher on surfaces of r=0.05 copolymerized hydrogels,sugges-ting that the incorporation ofγ-PGA could stimulate cell growth.In addition,L929 cells could spontaneously detach from the surface of the poly（γ-PGA-co-NIPAAm）hydrogels without treating with any enzymes,and the detached cells at 20 ℃ showed higher viability than those detached from that on PNIPAAm hydrogel and TCPS.
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