中文摘要：

目的：建立当药高效液相色谱（HPLC）特征图谱的质量评价方法。方法：采用Waters 2695-2996型高效液相色谱仪,Dionex Ultimate 3000型高效液相色谱仪,Agilent^（-1）260型高效液相色谱仪;资生堂CAPCELL PAK C18MGⅡ色谱柱（4.6 mm×250 mm,5μm）,波士顿Green ODS色谱柱（4.6 mm×250 mm,5μm）,菲罗门Gemini 5μC18110A色谱柱（4.6 mm×250 mm）,以甲醇-乙腈-0.05 mol·L^（-1）磷酸二氢钠溶液（0.1%磷酸溶液调节p H至3.9）（15∶5∶80）为流动相,检测波长268 nm,流速1.0 m L·min^（-1）。采用主成分相对保留时间比较分析法,对10批不同产地的当药HPLC特征图谱进行评价。结果：建立了当药的HPLC特征图谱,共标定了5个共有峰,10批当药共有峰的相对保留时间的RSD〈2.0%。结论：该研究建立的当药特征图谱特征性和专属性强,且方法快速、简便、可靠,可用于控制当药的质量。

英文摘要：

Objective： To develop a method of HPLC fingerprinting analysis for the quality evaluation of Swertiae Herba. Method： The Waters 2695-2996, Dionex Ultimate 3000 and Agilent-1260 high performance liquid chromatograph instrument equipped with Shiseido CAPCELL PAK C18 MG Ⅱ （4.6 mm ×250 mm, 5 μm） , Boston Green ODS （4.6 mm x250 ram, 5 μm） and Phenomenex Gemini C18 110A column （4.6 mm x250 mm, 5 μm） was used. Methanol- acetonitrile-0.05 mol · L-1 sodium dihydrogen phosphate solution （ adjusted pH value to 3.9 with 0. 1% phosphoric acid solution） （15 ： 5 ： 80） were used as mobile phase; the wavelength was set at 268 nm, and the flow rate was 1.0 mL · min-1. The principal component relative retention time comparison method was used to evaluate the characteristic fingerprints of 10 batches of Swertiae Herba produced in different regions. Result： The chromatographic fingerprint was completed with five common peaks, and the RSD of relative retention time for the common peaks from 10 batches of Swertiae Herba was less than 2.0%. Conclusion： The method is simple, accurate and sensitive, which can be used for the quality control of Swertiae Herba.