中文摘要：

目的探讨不同价态砷染毒雄性大鼠肾脏砷代谢产物与相关代谢酶之间的关系。方法Wistar雄性大鼠35只，体质量150～190g，按体质量采用随机数字表法分为7组，每组5只大鼠。其中亚砷酸钠低、中、高剂量组染砷（亚砷酸钠）剂量分别为2．2、6.7、20．0mg／kg；砷酸钠低、中、高剂量组染砷（砷酸钠）剂量分别为2．2、6.7、20．0mg／kg；对照组饮用去离子水。染砷3个月后，处死动物，取大鼠肾脏，-80℃冷冻保存。采用高效液相色谱．氢化物发生原子荧光光谱法（HPLC．HGAFS）检测大鼠。肾脏中各形态砷代谢产物，酶联免疫法检测并分析相关代谢酶的含量、活力。并探讨砷代谢产物与相关代谢酶之间的关系。结果各组大鼠肾脏总砷（TAs）、二甲基砷（DMA）、一甲基砷（MMA）、甲基转移酶活力组间比较差异有统计学意义（F值为1874．672，H值分别为33513、31．002，F值为79．607，P均〈0．01）。其中亚砷酸钠低、中、高剂量组TAs[（526．52±25．56）、（1654．00±101．55）、（1904．24±104．76）μg／kg]和DMA[（323．20±16．13）、（1444．40±113．81）、（1765．40±104．39）μg／kg]均高于砷酸钠相应各剂量组[（235．70±6．23）、（471．05±18．32）、（1677．40±83．29）μg／kg，（0．00±0．00）、（1．75±0．16）、（410．50±19．76）μg／kg，P〈0．0024或〈0．05]；亚砷酸钠低、中、高剂量组MMA[（4．02±0．86）、（4．20±0．65）、（4．04±0．80）μg／kg]均低于砷酸钠相应各剂量组[（98．90±9．59）、（376．50±15．41）、（1131．90±74．26）μg／kg，P均〈0．05]；亚砷酸钠低、中、高剂量组甲基转移酶活力[（7．80±0．93）、（5．55±0．49）、（3．56±0．26）U／g]均低于砷酸钠相应各剂量组[（11．59±0．93）、（8.93±0．88）、（6．52±1．04）U／g，P均〈0．0024]。亚砷酸钠低、中、高剂量组DMA?

英文摘要：

Objective To investigate the relationship between metabolites of sodium arsenite and sodium dihydrogen arsenate with related metabolic enzymes in kidney of male rats. Methods According to body mass, thirty-five male Wistar rats（body mass 150 - 190 g） were divided into 7 groups by random number table. Control group drank deionized water; the contents of iAs± in low, medium and high arsenite groups and the contents of iAsv in low, mediu .m and high of sodium dihydrogen arsenate groups were 2.2, 6.7 and 20.0 mg/kg, respectively. After 3 months, kidneys were collected and stored at - 80 ±C; high performance liquid chromatography and hydride genesis atomic fluorescence spectroscopy （HPLC-HGAFS） was used to determine the level of arsenic metabolites in kidney, and enzyme-linked immunosorbent assay was used to detect and analyze the content or the activity of metabolic enzymes, meanwhile correlation studies between the level of metabolites and the activity of metabolic enzymes were carried out. Results The differences of total arsenic （TAs）, dimethyl arsenic acid （DMA）, monomethyl arsenic acid （MMA） and methyl transferase enzyme activity in kidneys of rats between groups were statistically significant （F = 1874.672, H = 33.513, 31.002, F = 79.607, all P 〈 0.01）. The TAs[ （526.52 ± 25.56）, （1 654.00 ± 101.55）, （1 900.24 ± 100.76）μg,/kg] and DMA[（323.20 ± 16.13）, （1 444.40 ± 113.81 ）, （1 765.40 ± 100.39）μg/kg] of sodium arsenite in low, medium and high dose groups were higher than those of the corresponding sodium dihydrogen arsenate groups [ （235.70 ± 6.23）, （471.05 ± 18.32）, （1 677.40 ± 83.29）μg/kg, and（0.00 ± 0.00）, （1.75 ± 0.16）, （410.50 ± 19.76）μg/kg, P 〈 0.0024 or 〈 0.05]; the MMA[（4.02 ± 0.86）, （4.20 ± 0.65）, （4.04 ± 0.80）±g/kg] of sodium arsenite in low, medium and high dose groups were lower than those Of the corresponding sodium dihydrogen arsenate groups[ （98.90 ± 9.59）, （376.50 ± 15.41）, ?