中文摘要：

目的探讨硒、碘缺乏模型动物对F2代雌性、雄性生殖功能的影响。方法采用自制人工合成饲料，利用SD大鼠建立低硒、低碘及硒碘联合缺乏动物模型，确定动物模型建立成功后，利用该模型进行大鼠的三代繁殖试验及模型稳定性监测。观察F2代模型大鼠精子形态、血清睾酮、睾丸和附睾组织病理及透射电镜超微结构的改变；检测F2代模型孕鼠生育指标、胎仔骨骼及内脏发育指标，探讨硒、碘缺乏对F2代模型动物雌性、雄性生殖功能的影响。结果经各项指标检测，三代繁殖试验中动物模型稳定性良好。通过对雄性F2代模型动物大鼠的精子形态学指标、血清睾酮含量的检测及睾丸、附睾组织病理形态学、超微结构透射电镜观察，低硒、低碘及硒碘联合缺乏均可引起雄性F2代模型动物的生殖功能损伤。通过对雌性F2代模型动物妊娠鼠体重、胚胎发育及其F3代胎仔骨骼、内脏发育的检测，低硒、低碘及硒碘联合缺乏均可引起雌性F2代模型动物生殖功能损伤。结论低硒、低碘及硒碘联合缺乏均可引起F2代模型动物生殖功能损伤。

英文摘要：

Objective To observe the affection of selenium and iodine deficiency on the reproductive function of male and female F2 rat with the established model. Methods Artificial synthesize feeds was used in our research. The healthy SD rat ceased lactation were chosen and randomly divided into three groups of lower selenium, lower iodine and two elements deficiency group and then raised in divided cages. The reproductive test of three generation was taken after evaluating the stability of the model. Meanwhile the fertility impact on the male and female rats in F2 was studied by observing the changes on sperm morphology, serum biochemical indexes, testicle pathology, fertility indexes, embryo osteogenesis and the viscera development. Results The animal models of lower selenium, lower iodine and the two elements deficiency were established successfully. The stability of three animal models was satisfied during the experiment of three generation reproduce test. Deficiency of selenium, iodine and both of them can all impact the reproductive function of male rats on its morphological index of sperm, biochemical index of sperm and morphological and pathological changes in testicle. They can also impact the reproductive function of female rats on the weight of the second generation of pregnant rats, the embryonic and organic development of the third generation. Concision Deficiency of selenium and iodine can all impact the reproductive function on F2 model rats.