中文摘要：

植物病毒病给农业生产造成极大的损失。据统计，全世界每年仅烟草花叶病毒（tobacco moscaic virus，TMV）造成的损失就达1亿美元以上（吴云峰，1995）。植物是天然药物的宝库，国内外学者对植物中抗病毒活性物质进行了广泛深入的研究（侯玉霞等，2000；李重九等，1997；French et al．，1991；Fredrich et al．1996），发现了一些对TMV有较好效果的抑制剂。

英文摘要：

The antiviral substances of walnut leaves were extracted by different solvents and methods, and its antiviral activities were determined. The results showed that antiviral substance activity is dissimilar based on extracts from walnut leaves with different solvent and methods. Inhibition rate on TMV of the extract with 95% （V/V） ethanol is the best, 100% ; Optimum condition of the cold soak are： 95% （V/V） ethanol is used as solvent, the ratio of material to solvent is 20/100 （1：10）, soak time is 18 h. The inhibition rate of the extract to TMV infecting Nicotiana glutinosa is 100% under optimum condition.