环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)是一种新型的能在恒定温度条件下实现核酸序列快速扩增的技术.由于该技术具有快速、便捷、高效和低成本等优势,目前已被广泛应用于分子诊断方面.文中介绍了LAMP技术的原理、研究进展、分子检测方法,以及该技术在农业分子诊断与基因检测方面的应用.
Loop-mediated isothermal amplification(LAMP) is a new technique for rapid amplification of nucleic acid sequences under a constant temperature. Due to the advantages of the technique,such as rapdity,convenience,high efficiency and low cost,it has been widely used in molecular diagnosis. We introduce its principle,research advances,testing method and application of molecular diagnosis and gene detection in agriculture.