国家已禁止向小麦粉中添加过氧化苯甲酰(BPO),快速检测小麦粉中BPO添加量对于保障食品安全非常重要。BPO在添入小麦粉中后部分会被还原成为苯甲酸,使得对其原始添加量的检测非常繁琐。本文探讨了利用近红外漫反射光谱法进行小麦粉中BPO添加当量检测的可行性。通过向无添加剂小麦粉中添加不同量的过氧化苯甲酰粉末,制备小麦粉样本133个。样本在实验室放置一段时间后,采集光谱数据,通过小波变换滤除光谱噪声。用偏最小二乘法建立光谱数据与BPO原始添加当量之间的模型,模型校正集决定系数Rc2al是0.890 1,校正标准偏差RMSEC是40.85mg.kg-1,预测决定系数R2pred为0.886 5,预测标准偏差RMSEP为44.69mg.kg-1。结果表明:基于近红外光谱的小麦粉BPO添加当量检测具有较高的可行性,该方法有望为食品中某些添加物的快速检测提供新的途径。
Adding benzoyl peroxide(BPO)into wheat flour was prohibited by the relevant government departments since May 1,2011.And it is of great importance to detect BPO additive amount in wheat flour quickly and accurately.Part of BPO which wasadded into wheat flour will be deoxidized into benzoic acid,and this make it complex to detect the original BPO additive amount.The objective of the present research is to investigate the potential of NIR diffuse reflectance spectroscopy as a way for measure-ment of BPO original adding amount in wheat flour.A total of 133wheat flour samples were prepared by adding different contentof BPO into pure wheat flour.Spectra data were obtained by NIR spectrometer and then denoised by wavelet transform.Ninetyseven samples were taken as calibration set and other 36samples as prediction set.Partial least squares regression(PLSR)wasapplied to establish the calibration model between BPO original adding contents and the spectra data.The determination coeffi-cient of model for the calibration set is 0.890 1,and root mean squared error of calibration(RMSEC)is 40.85mg.kg-1.Thedetermination coefficient for the prediction set is 0.886 5,and root mean squared error of prediction(RMSEP)is 44.69mg.kg-1.The result indicates that it is feasible to detect the BPO adding contents in wheat flour by NIR diffuse reflectance spectros-copy technique and this technique has the potential to measure some other additives in food.