中文摘要：

目的：探讨丁酸钠对人外周血来源的树突状细胞（DC）的成熟状态和免疫学功能的影响。方法：通过粒细胞一巨噬细胞集落刺激因子（GM—CSF）和白细胞介素4（IL-4）结合丁酸钠体外诱导人外周血来源的DC，6d后结合不同成熟因子诱导成熟，并以流式细胞仪、FITC标记的Dextran的内吞检测、混合淋巴细胞反应（MLR）、ELISA法分别检测DC的表面标志、内吞能力、DC刺激淋巴细胞增殖能力和白细胞介素12（IL-12）分泌量的改变。结果：丁酸钠可以抑制DC成熟，使DC具有较强的抗原吞噬能力，而刺激淋巴细胞增殖能力和IL—12的分泌能力下降。结论：丁酸钠可以抑制DC成熟，诱导不成熟DC生成，在移植免疫耐受方面具有较好的应用前景。

英文摘要：

AIM： To investigate the immunological function of sodium butyrate - induced immature dendritic cells in vitro. METHODS ： The human monocyte - derived dendritic cells were induced in the presence of human granulocyte macrophage- colony stimulating factor（ GM -CSF） and interleukin- 4 （IL- 4）, combined with sodium butyrate. The immunological function of sodium butyrate - induced dendritic cells was detected by the FCM, endocytic activity, T cells stimulatory proliferation capacity, and interleukin - 12 （IL - 12） production. RESULTS： Sodium butyrate could down- regulate the major histocompatibility complex （MHC） class Ⅱ and costimulatory molecules of dendritic cells, increase the endocytic activity, induce a stage of T - cell anergey, and inhibit the T helper cell type 1 - skewing factor IL - 12 production. CONCLUSION： Sodium butyrate inhibits the maturation of dendritic cells and induces production of immature dendritic cells, which may help to explore the machenism of its epigenitic modification.