以杂交狼尾草为原料,采用CaO/MgO复合固体碱为催化剂,通过水相环境下高温处理,研究反应条件对狼尾草酶解效率及总糖收率的影响。研究结果表明:在催化剂含量20%,温度110℃,时间80min,固液比1:20的条件下预处理狼尾草,木质素去除率高达54.18%。加酶量为40FPU/g,酶解72h,葡聚糖收率从未处理时的41.23%增大到81.04%;CaO/MgO固体碱预处理可破坏杂交狼尾草原本的致密结构,暴露更多的纤维素,破坏纤维素中的分子内和分子间的化学基团。CaO/MgO固体碱循环使用4次后无明显失活。
The effects of the reaction conditions on enzymatic digestibility and total sugar yield of Pennisetum hybrid pretreated in high temperature aqueous environment with composite solid alkali-CaO/MgO as the catalyst are investigated in this paper. The results indicated that 20% catalyst content, 110 ℃, 80 min and 1 : 20 ratio of solid are the optimal pretreatment conditions, whose lignin removal rate is up to 54.18%. When the enzyme loading is 40 FPU/g, 72 h enzymatic digestibility increases from 41.23% to 81.04%, substrates can be fully hydrolyzed. After pretreatment, the compact structure is damaged, more fibers expose, the chemical groups are destroyed like hydrogen bond in molecular. The catalysts CaO/MgO can be recovered and regenerated.