中文摘要：

目的：通过检测表皮生长因子受体（EGFR）在喉癌组织标本不同部分的表达，定量分析EGFR表达与喉癌组织分化之间的关系。方法：收集36例喉癌组织标本，根据病理分级分为高、中、低分化3个组。运用WesternBlot方法检测EGFR在癌组织、癌旁组织及正常组织中的表达，结合图像分析技术定量分析EGFR表达与喉癌组织分化之间的关系。结果：不同分化的喉鳞状细胞癌组织、癌旁组织中EGFR相对表达量差异均有统计学意义（均P〈O．05）。正常组织在不同分化病例中EGFR相对表达量差异无统计学意义（P〉0．05），同一分化鳞状细胞癌组织、癌旁组织及正常组织间EGFR相对表达量差异有统计学意义（P〈0．05）。结论：EGFR在喉癌组织中有高表达，与癌组织的分化有相关性，对研究喉癌的发生发展有一定的临床意义。

英文摘要：

Objective： To detect the expression of EGFR in different region of laryngeal tissue,and use quantitive analysis to discuss the relation between expression of EGFR and histological differentiation. Method： Collected 36 cases of laryngeal tissue example, which be divided in to three groups based on pathobiology. Using Western Blot to detect the EGFR expression in cancer tissue, paracancer tissue and normal tissue,and combined imaging analytical technique to analyse the relation between expression of EGFR and histological differentiation. Result：In same region of cancer tissue the expression of EGFR is different along with different tissue differentiation（P〈 0.05） ,but in normal tissue this different is not existing（P〉0.05）. In same tissue differentiation the expression of EGFR is different in cancer tissue, paracancer tissue and normal tissue（P〈0.05）. Conclusion.. EGFR highly ex press in laryngeal cancer tissue, and relate with the tissue differentiation of laryngeal cancer. EGFR is an important indicator to study the emerging and progression of laryngeal cancer.