中文摘要：

针对成熟油菜次生休眠种子富含多糖和多酚、老健组织部分富含RNA酶、生理活性低等特点,通过选用RNAplant plus reagent植物总RNA提取试剂,添加β-巯基乙醇抑制RNA酶活性、防止酚类物质氧化,应用醋酸钠去除多糖类物质对RNA的影响,以及缩短抽提时间等措施,建立成熟油菜次生休眠种子RNA的快速高效提取方法。结果表明：采用本方法提取的RNA D260nm/D280nm为1.92～2.05,28SrRNA和18SrRNA条带清晰而完整,且无明显降解,可以应用于后续的mRNA分离、建库和高通量转录组测序。

英文摘要：

Isolation of high quality RNA is an essential prerequisite for gene expression analysis on rape seed develop- ment mechanism. The secondary dormant seeds of mature rapeseed were rich in polysaccharids and polyphenols, while the old tissues had high Rnase activity and low physiological activity. According to these characteristics, we developed a rapid and simple method for the isolation of total RNA through choosing RNA plant plus reagent DP437 （TIANGEN） and tak- ing some measures such as adding β-mercaptoethanol, NaAc and reducing the extraction times to inhibit RNA enzyme ac- tivity, preventing oxidation of phenolic compounds and removing the effects of polysaccharide substance respectively. Two clear bands of 28S rRNA and 18S rRNA isolated from rapeseeds could be detected by agarose gel electrophoresis. RNA extracted by this method was intact without degradation. The D260 nm/D280 nm absorbance ratio was 1. 92 -- 2.05. These results showed that the RNA obtained from rapeseed with this method had high quality and were suitable for the subsequent molecular studies, including mRNA separation, standard mRNA library constructing and high-throughput RNA-seg etc. At the same time this method would provide technical guarantee for exploring the molecular mechanism of secondary dormancy in rapeseed, and also could be used for isolation of total RNA from mature dormant seeds rich in pol- ysaccharides and polyphenols of other plants.