中文摘要：

大腹园蛛大壶状腺表达拖丝蛋白新基因的克隆，为进一步研究蛛丝蛋白基因以及人工表达蛛丝蛋白提供参考依据。文章利用“通用方法”即反转录-置换法构建大腹园蛛（Araneus ventricosus）大壶状腺（Major ampullate gland）cDNA文库，并筛选出具有典型重复结构的大腹园蛛大壶状腺丝蛋白-1部分cDNA序列AvMaSpⅠ（GenBank登录号：AY177203）。该部分序列大小为1408bp，编码区为1288bp，编码氨基酸429个，预测分子量为34．07kDa，典型的重复结构为（GA）nAm（GA）N，与十字园蛛（Araneus diadematus）丝蛋白基因ADF-1（GenBank登录号：ADU47853）同源关系最近，一致性为75．0％。

英文摘要：

In this paper, we successfully constructed the cDNA library of major ampullate gland ofAraneus ventricousus using pUC18 vector and cloned the partial cDNA （AvMaSp1, GenBank accession number AY177203） encoding spider major ampullate gland spidroin-1 by means of picking colony randomly （Bird gun）. The partial cDNA sequence of AvMaSp1 was 1 408 bp and its region in encoding was 1 288 bp. The protein deduced from AvMaSpl contained 429 amino acid residues and molecular weight was 34.07 kDa. The repetitive motif of this cDNA sequence was （GA）nAm（GA）N and the identity with A. diadematus fibroin-1 mRNA （ADF-1, GenBank accession number ADU47853） was 75.0%.