中文摘要：

目的：建立SD大鼠舌黏膜鳞癌细胞系（sprague-dawley rat tongue mucosa squamous cell carcinoma cell line ，Rca-T），并观察其生物学特性，为口腔癌的基础研究提供大鼠来源的恶性细胞系及荷瘤动物模型。方法：将4-硝基喹啉-1-氧化物诱发的SD大鼠舌黏膜鳞癌组织进行原代培养，并用有限稀释法获得单克隆细胞。光学显微镜观察细胞的形态，CCK-8法检测细胞的增殖能力，流式细胞仪检测其细胞周期，动物实验观察细胞裸鼠皮下成瘤率。结果：成功获取大鼠舌黏膜鳞癌单克隆细胞系，细胞呈梭形，群体倍增时间为23．35 h，平板克隆形成率为63．06％，CK、Vimentin和N-cadherin免疫组织化学染色均为阳性，细胞系裸鼠皮下接种成瘤率为100％（6/6）。结论：成功建立大鼠舌黏膜鳞癌单克隆细胞系Rca-T，可作为研究口腔鳞癌发生机制和诊断治疗的细胞模型。

英文摘要：

Objective：To establish a monoclone cell line of squamous cell carcinoma （SCC）in rat tongue mucosa and to study its bi-ological characteristics.Methods：SCC in rat oral mucosa was induced by adding 4-nitroquinoline-1-oxide （4NQO） to the SD rat′s drinking water , and the cancer tissue was then cultured to obtain mixed cells in vitro .Mono cell cloning method was used for purifica-tion of the mixed tumor cells .The biological characteristics of the cells were studied by microscope observation , CCK-8 Cell Counting Kit, plate clone formation assay , flow cytometry assay and immunohistochemistry staining .Hypodermic inoculations of the cells in nude mice were performed to observe the tumor formation ability .Results：The tumor cells were spindle-shaped and expressed the proteins of cytokeratin, Vimentin and N-cadherin.The cellular population doubling time was 23.35 hours.The colony forming efficiency was 63.06%and the xenograft rate was 100% in nu/nu BALB/C mice.Conclusions：Rca-T, an oral squamous cell carcinoma cell line derived from Sprague-Dawley rat tongue mucosa carcinoma , was established successfully .This cell line would be a model for basic and clinical research of oral cancer .