中文摘要：

从华石斛（Dendrobium sinense T.Tang et F.T.Wang）根部中分离得到1株真菌S1。通过显微镜观察菌丝形态,PCR扩增ITS-5.8S r DNA序列,测序并在NCBI中进行同源比较,确定其种类;采用高效液相色谱法对该菌株发酵液中的游离氨基酸进行检测。结果表明：根据形态学和分子生物学鉴定的方法,将从华石斛分离的S1菌株鉴定为胶膜菌科（Tulasnellaceae）胶膜菌属（Tulasnella sp.）真菌,该菌在PDB和DE发酵液中均检测到17种氨基酸,其中脯氨酸（Pro）、半胱氨酸（Cys）和赖氨酸（Lys）3种氨基酸含量远高于其他氨基酸的含量。

英文摘要：

The strain S1 was separated from the roots of Dendrobium sinense, its mycelial morphology was observed under the microscope, and the ITS-5.8S rDNA sequences of the strains were cloned, sequenced and analyzed by using BLAST. The strain isolated was cultured in PDB and DE liquid culture media, and plant free amino acids were detected by using high performance liquid chromatography （HPLC）. The strain S1 isolated from the roots of D. sinense was identified as Tulasnella sp. of the family Tulasnellaceae, and detected to contain 17 essential amino acids in both PDB and DE fermentation broths, of which 3 amino acids, Pro, Cys and Lys, were much higher than the other amino acids.