中文摘要：

黑色素瘤是一种常见的恶性肿瘤，随着恶性程度的增高，黏着斑激酶（FAK）水平递增。本文利用RNAi技术，成功建立了稳定干扰FAK基因的F10-siFAK及阴性对照F10-control两种细胞系。与对照F10-control相比，F10-siFAK细胞系mRNA表达下降，蛋白表达减少，ERK磷酸化显著降低，细胞周期在G1期阻滞增加，而且在动物体内的生长速度明显降低。稳定干扰FAK基因的黑色素瘤细胞株的建立，为研究FAK在黑色素瘤进程中的作用提供了工具，并为以FAK为靶点的黑色素瘤治疗药物的筛选提供了平台。

英文摘要：

Malignant melanoma still remains to be a serious health threat. Overexpression of focal adhesion kinase （FAK） in melanoma has suggested that FAK could be a promising target for therapeutic intervention. To further investigate the function of FAK in melanoma, FAK expression was down-regulated by stable transfection of plasmid harboring FAK small interfering RNA （siRNA） into melanoma cell line. Two stable cell lines, F10-siFAK and F10-control, have been constructed and screened. Compared with the F10-control, both the mRNA and protein levels of FAK decreased significantly, and the cell cycle of F10-siFAK was arrested at G1 phase. Furthermore, the tumor growth rate of F10-siFAK cells was notably slower than that of F10-control in in vivo tumor models. These results show that FAK is an important regulatory gene in melanoma. The stable FAK-knockdown melanoma cell line is an useful tool for further investigation of FAK＇s function in the progression of melanoma, and also an effective means of drug screening for anti-melanoma therapeutics.