中文摘要：

密度坡度 centrifugation 从外部血孤立的 Mononuclear 房间(MNC ) 是人的 fibronectin 涂的文化盘子上的 plated 并且在 EGM-2 有教养中等。依附塑造锭子的房间，由一些调查者作为 endothelial 祖先房间(EPC ) 报导了，从支持者回合房间伸长了，但是没从以前想了的房间的一个小数字增殖。主要 EPC 的生长曲线证明房间几乎没有很少 proliferative 能力。当他们能也表示 CD14 时，流动 cytometry 分析证明房间能表示一些 endothelial 系标记，它在整个文化被认为单核白血球 / 巨噬细胞系的一个标记。在 endothelial 函数试金，房间比在反向的抄写聚合酶的成熟 endothelial 房间锁住反应并且没显示一个能力在 vitro 在 angiogenesis 试金开发像试管的结构表明了 eNOS 的表示的底层。在这研究，我们由低密度的脂蛋白(Dil-Ac-LDL ) 和印度墨水举起测试的联合标记 Dil 的乙酰 ated 识别了 EPC 的单音的似细胞的功能。所有房间是双的为在天的 Dil-Ac-LDL 和印度墨水举起积极 4 文化， 14 和 28，它意味着 EPC 在整个文化维持了单音的似细胞的功能。因此，尽管从外部 MNC 的成年 EPC 有一些 endothelial 系性质，他们维持典型 monocytic 功能并且几乎没有很少 proliferative 能力。

英文摘要：

Mononuclear cells （MNCs） isolated from peripheral blood by density gradient centrifugation were plated on human fibronectin-coated culture plates and cultured in EGM-2 medium. Attached spindle-shaped cells, reported as endothelial progenitor cells （EPCs） by some investigators, had elongated from adherent round cells, but had not proliferated from a small number of cells as supposed previously. The growth curve of the primary EPCs showed that the cells had little proliferative capacity. Flow cytometry analysis showed that the cells could express some of the endothelial lineage markers, while they could also express CD 14, which is considered a marker of monocyte/macrophage lineages throughout culture. In endothelial function assays, the cells demonstrated a lower level of expression of eNOS than mature endothelial cells in the reverse transcription-polymerase chain reaction and did not show an ability to develop tube-like structures in angiogenesis assay in vitro. In this study, we identified the monocytoid function of EPCs by the combined Dillabeled acetylated low-density lipoprotein （Dil-Ac-LDL） and Indian ink uptake tests. All the cells were double positive for Dil- Ac-LDL and Indian ink uptake at days 4, 14 and 28 of culture, which means the EPCs maintained monocytoid function throughout the culture. Therefore, although adult EPCs from peripheral MNCs have some endothelial lineage properties, they maintain typical monocytic function and have little proliferative capacity.