中文摘要：

背景 hepatopulmonary 症候群(HPS ) 是在与肝硬化和门高血压在病人导致全身的血氧不足的肺的严重脉管的复杂并发症。在 HPS 的内在的结构的变化是 intrapulmonary vasodilation，它能导致肺的静脉的血的损害氧化。heme oxygenase-1/carbon 一氧化物(HO-1/CO ) 系统在脉管的音调的控制起一个重要作用，这被表明了。这研究的目的是进一步完全在动物 model.Methods 在 HPS 的致病调查 HO-1 的角色 35 只老鼠被划分成肝肝硬化，锌 protoporphyrin (ZnPP ) ，钴 protoporphyrin (CoPP ) 和假冒的组。胆汁的肝硬化被胆汁管结扎在开始的三个组建立。在 ZnPP 和 CoPP 组的老鼠收到了 ZnPP 和 CoPP 的 intraperitoneal 注射一次分别地，在在肺的 HO-1 mRNA 的样品 collection.Expression 前的 24 个小时被反向抄写的聚合酶链反应检测，当蛋白质表示被 immunohistochemical 分析决定时。Hematoxylin 并且曙红染色被执行证实肝肝硬化和 intrapulmonary vasodilation 的存在。动脉的血气体，吝啬的动脉的压力和门静脉压力也被测量。变化或 Wilcoxon 的分析统计方法被用来与假冒的组，相比决定统计 significance.Results 肝脏硬化症的组表明了肺的 HO-1 mRNA 和蛋白质(P0.01 ) 的增加的表示。动脉的 carboxyhemoglobin (COHb ) 的水平，牙槽动脉的氧坡度(A-aPO2 ) ，吝啬的动脉的压力，门静脉压力(P0.05，分别地) ，并且 intrapulmonary vasodilation 显著地也被增加。与肝脏硬化症的组相比， CoPP 处理增加了肺的 HO-1 mRNA 和蛋白质表示， A-aPO2 的水平( P 0.05 分别地)， COHb ( P 0.01 )，和 intrapulmonary vasodilation ，当 ZnPP 处理减少了时肺的 HO-1 mRNA 和蛋白质表示， COHb 的水平( P 0.05 分别地)，并且 intrapulmonary vasodilation ，没有吝啬的动脉的压力和门静脉 pressure.Conclusion 的明显的改变，增加的肺的 HO-1 表示是到develop 的一个重要贡献者

英文摘要：

Background The hepatopulmonary syndrome （HPS） is a severe vascular complication in lungs resulting in systemic hypoxemia in patients with cirrhosis and portal hypertension. The underlying structural change in HPS is intrapulmonary vasodilation, which can lead to impaired oxygenation of pulmonary venous blood. It has been demonstrated that the heme oxygenase-1/carbon monoxide （HO-1/CO） system plays an important role in the control of vascular tone. The aim of this study was to further investigate the role of HO-1 in the pathogenesis of HPS in animal model.Methods Totally 35 rats were divided into liver cirrhosis, zinc protoporphyrin Ⅸ （ZnPP）, cobalt protoporphyrin （CoPP）and sham groups. Biliary cirrhosis was established in the first three groups by bile duct ligation. Rats in the ZnPP and CoPP groups received once intraperitoneal injection of ZnPP and CoPP, respectively, 24 hours before sample collection.Expression of HO-1 mRNA in lung was detected by reverse-transcription polymerase chain reaction, while protein expression was determined by immunohistochemical analysis. Hematoxylin and eosin staining was performed to confirm the presence of liver cirrhosis and intrapulmonary vasodilation. Arterial blood gases, mean arterial pressure and portal vein pressure were also measured. Analysis of variance or Wilcoxon statistical methods were used to determine statistical significance.Results Compared with the sham group, the cirrhotic group demonstrated increased expression of pulmonary HO-1 mRNA and protein （P〈0.01）. The level of arterial carboxyhemoglobin （COHb）, alveolar-arterial oxygen gradient （A-aPO2）,mean arterial pressure, portal vein pressure （P〈0.05, respectively）, and intrapulmonary vasodilation were also significantly increased. Compared with the cirrhotic group, CoPP treatment increased pulmonary HO-1 mRNA and protein expression, the level of A-aPO2 （P 〈0.05 respectively）, COHb （P 〈0.01）, and intrapulmonary vasodilation, while ZnPP treatment decreased pulmona