中文摘要：

为探寻高粱醋中皂苷的提取工艺以及家兔血小板聚集的影响,选用了超滤膜技术、有机溶剂萃取技术、葡聚糖凝胶分离技术,将高粱醋提取、分离、纯化得到的皂苷提取物。结果表明,在有机溶剂萃取过程中使用75%vol乙醇、超声提取30min、温度为50℃时,在葡聚糖凝胶层析过程中使用去离子水作为流动相,得到纯度较高的皂苷提取物。并使用ADP与胶原作为诱导剂,以兔血为血源,得到高粱醋中皂苷提取物具有很强的抗血小板聚集功能,且以ADP为诱导剂的IC50为（2.12±0.34）mg/mL、以胶原为诱导剂的IC50为（2.98±0.12）mg/mL。并在皂苷提取物中定量检测总皂苷含量,通过SPSS数据分析得到,皂苷提取物中总皂苷的含量与其抑制抗血小板聚集活性的相关性成立（p〈0.01）。可知山西高粱醋中皂苷对抑制家兔血小板聚集有较强活性。

英文摘要：

To explore extraction process of saponin and the influence of functionality in sorghum vinegar on rabbits platelet aggregation,by adopting the technologies of ultrafiltration membrane,organic solvent extraction and sephadex extraction to separate and concentrate sorghum vinegar,obtains saponin extract.The results showed that the high purity of saponin extract could be produced by the following condition： 75%vol alcohol,30min,50℃ extract by ultrasonic and deionized water be the moving-phase.By using ADP and collagen as inducers and rabbit blood as blood source,it can be proved that soponin extract showed strong anti-platelet aggregation function,with IC50being（2.21±0.34） mg/ml for ADP as the inducer and（2.98±0.12） mg/ml for collagen as the inducer.Moreover,through a quantitative detection of saponin in saponin extract,data analysis of SPSS indicates that the content of saponin in saponin extract is correlated to its anti-platelet aggregation activity（p0.01）.Therefore,it can be concluded that saponin substances in Shanxi sorghum vinegar have strong activity in anti-platelet aggregation of rabbit.