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中文摘要：
An experimental and statistical study was carried out to explore the effects of mechanical forces on the ends of linear double-stranded DNA (dsDNA) fragments. Mechanical force was applied onto individual DNA molecules during atomic force microscope (AFM)-based picking-up manipulation. By comparing the PCR efficiency of two DNA fragments with primers either at ends or at the inner regions, it was found that the ends of DNA fragments were damaged during picking-up process.
英文摘要：
An experimental and statistical study was carried out to explore the effects of mechanical forces on the ends of linear double-stranded DNA （dsDNA） fragments. Mechanical force was applied onto individual DNA molecules during atomic force microscope （AFM）-based picking-up manipulation. By comparing the PCR efficiency of two DNA fragments with primers either at ends or at the inner regions, it was found that the ends of DNA fragments were damaged during picking-up process
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