中文摘要：

目的观察升清和健脾理气药物对饮食诱导肥胖（DIO）大鼠肥胖程度及对脂联素和炎症因子的影响。方法Wistar大鼠120只，10只作为空白对照组，给予基础饲料，其余110只给予高脂高营养饲料17周，按照体重，得到DIO大鼠40只和肥胖抵抗（DIO—R）大鼠10只，将DIO大鼠又分为DIO模型组、西布曲明组、健脾理气组和升清组，每组10只，分别以生理盐水（2mL／d）、西布曲明1．6mg／（kg·d）、健脾理气药物3．2g／（kg·d）、升清药物3．2g/（kg·d）灌胃，空白对照组与DIO—R组予生理盐水（2mL／d）灌胃。灌胃期间空白对照组予基础饲料，余5组继续高脂饲料。灌胃16周后，测量体重、身长，取腹腔内全部脂肪，测量肥胖程度及脂肪含量。取血测定胰岛素抵抗指数（IRI）、血糖、甘油三酯、胆固醇、肿瘤坏死因子（TNF—α）、脂联素。取脂肪匀浆测定TNF—α、脂联素。结果DIO模型组比正常大鼠体重、体重指数、脂肪系数、胆固醇、IRI明显升高（P〈0．05，P〈0．01）；血清和脂肪中脂联素均下降（P〈0．05），TNF—α均升高（P〈0．05，P〈0．01）。与DIO模型组比较，DIO—R组体重、体重指数、脂肪系数、IRI均降低（P〈0．05，P〈0．01）；血清和脂肪中脂联素升高（P〈0．01），TNF—Ot降低（P〈0．05，P〈0．01）。健脾理气药物可降低脂肪匀浆中TNF—α水平（P〈0．05）；升清药物可以升高血清及脂肪匀浆中脂联素水平，降低TNF-α（P〈0．05，P〈0．01）。结论升清药物能抑制高脂饲料诱导的肥胖和胰岛素抵抗，其机制与促进脂联素分泌，降低TNF—α水平有关。“脾不升清”可能是肥胖的病机关键之一。

英文摘要：

Objective To investigate the effects of Shengqing formula and with diet-induced obesity （DIO） and influence on adipon Methods 10 of 120 Wistar male rats were chosen as blank gro rats were fed with high-fat and high-nutrition diet for 17 weeks. ectin and Jianpi Liqi formula on the rat given inflammatory factors. up（i. g. saline 2 mL/d） , the other 110 According to body weights, 40 DIO rats and 10 DIO-resistence （DIO-R） rats were obtained. Then, DIO rats were randomly divided into four groups： model group（ i. g. saline 2 mL/d）, sibutramine group（ i. g. 1.6 mg/kg）, Jianpi Liqi group（ i. g. 3.2 g/kg） and Shengqing group（i, g. 3.2g/kg） ;while DIO-R group rats were i.g. saline 2 mL/d. During the 16-week-intragastic administration, blank control group was fed with basic diets, and the other five groups were still fed with high-fat diets. After 16 weeks, body weight, body length and all fat removed from abdomen were ＇measured to evaluate the level of obesity and fat content. Serum concentration of glucose, triglycerides, cholesterol, tumor necrosis factor （TNF-a） and adiponectin were tested, followed by insulin resistance index （IRI） calculated. Levels of TNF- αand adiponectin in abdominal adipose tissue were tested. Results Compared with blank group, in model group, body weight, Lee＇ s index, fat coefficient index, concentration of cholesterol, levels of TNF-α both in plasma and adipose tissue, and IRI significantly increased （P 〈 0.05,P 〈 0.01 ）. However, level of adiponectin in serum and adipose tissue decreased significantly （P 〈 0.05 ）. Meanwhile, compared with mode group, in DIO-R group, body weight, Lee＇ s index, fat coefficient index, and IRI all decreased （P 〈 0.05, P 〈 0.01 ） ; both levels of adiponectin increased （P 〈 0.01 ）, TNF-α decreased （ P 〈 0.05, P 〈 0.01 ）. Jianpi Liqi formula decreased the level of TNF-α in adipose tissue, and Shengqing formula increased lev- els of adiponectin and decreased those of TNF-α both in ser