中文摘要：

目的优选灯盏乙素的柱层析纯化条件。方法采用高效液相色谱法检测纯度，考察了不同固定相和流动相对灯盏乙素分离效果的影响。结果灯盏乙素的最佳纯化工艺条件为：固定相200-300目的硅胶柱，流动相V（CH：C1：）：V（CH30H）=5：1，常压洗脱。结论该方法操作简单，分离效果好，使用成本低。 X

英文摘要：

To optimize the column chromatography methods to sublimate the scutellarin. Methods The conditions of column chromatography were studied by investigating into the purity and yield rates of scutellarin. A high performance liquid chromatography assay was used for measurements. Results The optimal operation conditions of column chromatography separation were： the 200-300 mesh silica gel can be chosen as the stationary phase, the mixture ofCH_2CI_2 ： CH3OH =5 ： 1 （by volume） is the suitable mobile phase. Conclusion The results showed that this method was simple and effective.