中文摘要：

【目的】本研究旨在探索松墨天牛Monochamus ahernatus Hope中昆虫表皮蛋白（ICP）基因在幼虫各组织中和不同发育阶段的时空表达模式。【方法】用cDNA末端快速扩增方法（rapid amplification of cDNAends，RACE）克隆了松墨天牛表皮蛋白基因，用实时荧光定量PCR分析了该基因在幼虫体内和不同虫态中的表达。【结果】克隆获得的松墨天牛幼虫表皮蛋白基命名为MoallCP（GenBank登录号：AGX00998．1），开放阅读框长408bp，编码135个氨基酸，推测得到的蛋白的分子量为14．51kDa。由MoallCP推导的蛋白与桑天牛Apriona germariICP（AAM66718．1）氨基酸序列一致性为79％；与丽蝇蛹集金小蜂Nasonia vitripennisICP（NP-001161297．1）、果蝇Drosophila mojavensisICP（XP_002005461．1）、玉带凤蝶Papilio polytesICP（BAMl8876．1）等19种昆虫表皮蛋白的氨基酸序列一致性在35％～45％之间；在第10—26位氨基酸位处含有一个跨膜片段。MoallCP在幼虫头、体壁、脂肪体、血细胞、中肠和马氏管均有表达；在蛹和成虫中的表达量分别是在幼虫中的44％和161％。【结论】MoallCP与其他昆虫有较高的氨基酸序列一致性。MoallCP在松墨天牛幼虫内广泛表达；在各个发育阶段中，以成虫中的表达量最高。本文为进一步研究松墨天牛表皮蛋白基因的生理功能和松墨天牛的表皮化学奠定基础。

英文摘要：

[ Aim ] This study aims to explore the expression profiles of insect cuticle protein （ICP） gene in different larval tissues and developmental stages of Monochamus alternates. [ Methods ] The genecoding for cuticle protein of M. alternatus was cloned by rapid amplification of cDNA ends （RACE） method. Real-time quantitative PCR was used to detect the gene expression profiles. [ Results ] Thecloned cuticle protein gene from M. alternatus was named as MoallCP （GenBank accession no. AGX00998.1 ）. The opening reading frame of MoallCP is 408 bp in length, encoding a protein of 135amino acids with an estimated molecular mass of 14.51 kDa. Multiple sequence alignment indicated that MoalICP shares the highest amino acid sequence identity （79%） with ICP from Apriona germari（ AAM66718.1 ） , and 35% - 45% identities with ICPs from 19 insects including Nasonia vitripennis （ NP_001161297.1 ）, Drosophila mojavensis （ XP_002005461.1 ）, and Papilio polytes （ BAM18876.1 ）at the amino acid level. There is a transmembrane segment between the 10th and the 26th amino acid sites in MoalICP. MoallCP gene was expressed in head, body wall, fat body, haemocytes, Malpighiantubules, and midgut body of larvae. The relative expression level of MoallCP gene in pupae and adults was 44% and 161% of that in larvae, respectively. [ Conclusion] MoalICP has high amino acidsequence identity with ICPs from other insects. The MoallCP gene is expressed widely in M. alternatus larvae, and has the highest expression level in adults. The present study provides reference for furtherresearch on functions of MoallCP and epidermis chemistry of M. alternatus.