中文摘要：

背景增加了航线的增长光滑的肌肉房间(ASMC ) 在气喘的病人被观察，吸烟能在气喘加速 ASMC 的增长。阐明导致这些变化的分子的机制，我们在 ASMC 和 cyclin D1 的表示的增长上在 vitro 学习了香烟烟摘录(CSE ) 的效果，重要规章的蛋白质在从 8 只气喘的布朗挪威老鼠有教养的房间 cycle.Methods ASMC 含有被学习。在经过 3 和 6 之间的房间在学习被使用并且被划分成控制组， pcDNA3.1 组，组， CSE 组， CSE+pcDNA3.1 组和 CSE+pcDNA3.1-ascyclin D1 为干预基于条件组织的 pcDNA3.1-antisense cyclin D1 (ascyclin D1 ) 。ASMC 的增长与房间周期分析， MTT 比色的试金和增殖的房间被检验染色的原子抗原(PCNA ) immunocytochemical。 cyclin D1 的表示被反向的 transcriptase-PCR ( RT-PCR )和 S+G2M 阶段，在 490 nm 波长( A490 )的吸收度价值和在 CSE 组的 PCNA 蛋白质的表示率的百分比是的西方的 blotting.Results ( 1 )检测( 31.22 1.17 )%， 0.782 0.221 ，( 90.2 7.0 )%分别地，它显著地控制组与那些相比被增加(( 18.36 1.02 )%， 0.521 0.109 ，并且( 54.1 3.5 )%，分别地)( P < 0.01 )。在有为 30 个小时， S+G2M 阶段的百分比， A490 和 PCNA 的表示率的 antisense cyclin D1 plasmid 的 transfection 以后，在 ASMC 的蛋白质比在未经治疗的房间低得多(P < 0.01 ) 。(2 ) 在 CSE 的 cyclin D1 mRNA 的 A490 的比率组织是 0.288 0.034，它显著地与控制组的相比被增加(0.158 0.006 )(P < 0.01 ) 。在有为 30 个小时的 antisense cyclin D1 plasmid 的 transfection 以后，在 ASMC 的 D1 mRNA 多是的 cyclin 的 A490 的比率比在未经治疗的房间降低(P < 0.01 ) 。(3 ) 在 CSE 的 cyclin D1 蛋白质表示的 A490 的比率组织是 0.375 0.008，它显著地与控制组的相比被增加(0.268 0.004 )(P < 0.01 ) 。在有为 30 个小时的 antisense cyclin D1 plasmid 的 transfection 以后， cyclin D1 的 A490 的比率在 ASMC 的蛋白质表示比在未经治疗的房间低

英文摘要：

Background Increased proliferation of airway smooth muscle cells （ASMCs） are observed in asthmatic patients and smoking can accelerate proliferation of ASMCs in asthma. To elucidate the molecular mechanisms leading to these changes, we studied in vitro the effect of cigarette smoke extract （CSE） on the proliferation of ASMCs and the expression of cyclin D1, an important regulatory protein implicated in cell cycle. Methods ASMCs cultured from 8 asthmatic Brown Norway rats were studied. Cells between passage 3 and 6 were used in the study and were divided into control group, pcDNA3.1 group, pcDNA3.1-antisense cyclin D1 （ascyclin D1） group, CSE group, CSE＋pcDNA3.1 group and CSE＋pcDNA3.1-ascyclin D1 group based on the conditions for intervention. The proliferation of ASMCs was examined with cell cycle analysis, MTT colorimetric assay and proliferating cell nuclear antigen （PCNA） immunocytochemical staining. The expression of cyclin D1 was detected by reverse transcriptase-PCR （RT-PCR） and Western blotting. Results （1） The percentage of S＋G2M phase, absorbance value at 490 nm wavelength （A490） and the expression rate of PCNA protein in CSE group were （31.22±1.17）%, 0.782±0.221, （90.2±7.0）% respectively, which were significantly increased compared with those of control group （18.36±1.02）%, 0.521±0.109, and （54.1±3.5）%, respectively） （P 〈0.01）. After the transfection with antisense cyclin D1 plasmid for 30 hours, the percentage of S＋G2M phase, A490 and the expression rate of PCNA protein in ASMCs were much lower than in untreated cells （P 〈0.01）. （2） The ratios of A490 of cyclin D1 mRNA in CSE group was 0.288±0.034, which was significantly increased compared with that of control group （0.158±0.006） （P 〈0.01）. After the transfection with antisense cyclin D1 plasmid for 30 hours, the ratios of A49o of cyclin D1 mRNA in ASMCs was much lower than in untreated cells （P 〈0.01）. （3） The ratios of A490 of cyclin D1 protein expressi