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Mitochondrial DNA T7719G in tRNA-Lys gene affects litter size in Small-tailed Han sheep
  • 时间:0
  • 分类:Q959.468[生物学—动物学] Q78[生物学—分子生物学]
  • 作者机构:[1]College of Animal Science and Technology, China Agricultural University, Beijing 100193, China., [2]Institute of Animal Science and Veterinary of Hebe Province, Baoding 071000, China.
  • 相关基金:supported by the Project of Science and Technology of Hebei Province(15226308D); the National Key Basic Research Program of China(2014CB138500)
中文摘要:

在农场动物,经济性能上的 mitochondrial DNA (mtDNA ) 效果为繁殖和基因选择仍然保持热话题。这里,小尾巴的汉绵羊的 53 个母亲的系被用来调查 mitochondrial DNA 变化和编码发现的区域的 mitochondrial 扫的 lambing 崽 size.ResultsSequence 的协会 31 个非同义的变化,并且协会学习表明在 mtDNA tRNA-Lys 基因的 T7719G 与崽尺寸被联系( P < 0.05 ),每在 G 和 T 搬运人之间的垃圾表明 0.29 个羔羊。而且,使用混合线性模型,我们 assayed 包括一般 haplotypes ,羊似的崽尺寸和 haplogroups 和多重水平的 mtDNA haplotypes 的潜在的协会装配了包含的运输链定序的电子(H等等)的 haplotypes , mitochondrial 呼吸复杂包含的序列( H-MRC )和 mitochondrial 基因(H基因,包括编码多肽的基因, rRNA 基因和 tRNA 基因)。为装配 mitochondrial haplotypes 的策略在经济特点上在 mtDNA 协会分析第一次被建议,尽管任何一个都不能重要关系被结束(P > 0.05 ) 。另外,原子主要基因 BMPR1B 显著地在成群与崽尺寸被相关( P < 0.05 )然而,没与 mtDNA T7719G 变化交往( P > 0.05 )编码基因的羊似的 mitochondrial 的 .ConclusionsOur 结果热点变化,在 tRNA-Lys 基因建议 T7719G 是为绵羊崽 size.Electronic 的选择的一个潜在地有用的标记这篇文章( doi:10.1186/s40104-017-0160-x )的联机版本包含的增补 materialThe 增补材料,它对可得到

英文摘要:

Background: In farm animals, mitochondrial DNA(mtDNA) effect on economic performance remains hot-topic for breeding and genetic selection. Here, 53 maternal lineages of Small-tailed Han sheep were used to investigate the association of mitochondrial DNA variations and the lambing litter size.Results: Sequence sweeping of the mitochondrial coding regions discovered 31 non-synonymous mutations, and the association study revealed that T7719G in mt DNA t RNA-Lys gene was associated with litter size(P 〈 0.05),manifesting 0.29 lambs per litter between the G and T carriers. Furthermore, using the mixed linear model, we assayed the potential association of the ovine litter size and haplogroups and multiple-level mtDNA haplotypes,including general haplotypes, assembled haplotypes of electron transport chain contained sequences(H-ETC),mitochondrial respiratory complex contained sequences(H-MRC) and mitochondrial genes(H-gene, including polypeptide-coding genes, rRNA genes and tRNA genes). The strategy for assembled mitochondrial haplotypes was proposed for the first time in mtDNA association analyses on economic traits, although none of the significant relations could be concluded(P 〉 0.05). In addition, the nuclear major gene BMPR1B was significantly correlated with litter size in the flock(P 〈 0.05), however, did not interact with mtDNA T7719G mutation(P 〉 0.05).Conclusions: Our results highlight mutations of ovine mitochondrial coding genes, suggesting T7719G in tRNA-Lys gene be a potentially useful marker for selection of sheep litter size.

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