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酶联免疫分析技术研究进展
  • 期刊名称：分析科学学报，2011,27(1):123-128
  • 时间：0
  • 分类：Q503[生物学—生物化学]
  • 作者机构：[1]南京大学医药生物技术国家重点实验室,南京大学,江苏南京210093
  • 相关基金：国家自然科学基金（No.30870588）;国家自然科学基金创新研究群体项目（No.30821006）
  • 相关项目：PPARγ调控的氧化还原在肝细胞癌性别差异中的作用及机制
关键词： ELISA, 固相化, 封闭, 灵敏度, Enzyme-linked immunosorbent assay, Immobilization, Block, Sensitivity
中文摘要：

酶联免疫吸附分析（ELISA）由于检测性能上的优越性使得其在医疗卫生、环境污染控制、食品安全监测等领域得以广泛应用,并且随着其他学科新技术的引进以及ELISA本身关键技术上的突破,ELISA的分析能力较以前有了巨大进步。本文综述了近年来在提高ELISA分析的敏感性、特异性以及稳定性等方面所采取的改进策略和方法,并对其发展方向进行了展望。

英文摘要：

Due to the advantages in detection performance,enzyme-linked immunosorbent assay（ELISA） has been widely used in the disciplines of health care,control of environmental pollution,monitoring of food safety and so on.With the introduction of new technologies as well as key technological breakthroughs,tremendous progress in its analytical capacity has been made.In this paper,we will review the application of improved strategies and methods for improving the sensitivity,specificity,and stability of ELISA analysis in recent years.

同期刊论文项目
天然药源分子及其新作用特点
期刊论文 41 获奖 1
PPARγ调控的氧化还原在肝细胞癌性别差异中的作用及机制
期刊论文 10 会议论文 2
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