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不同表面积和不同温度的热灸样刺激对大鼠延髓背侧网状亚核神经元的激活作用
  • ISSN号:1000-0607
  • 期刊名称:《针刺研究》
  • 时间:0
  • 分类:Q424[生物学—神经生物学;生物学—生理学]
  • 作者机构:[1]中国中医科学院针灸研究所,北京100700
  • 相关基金:国家重点基础研究发展计划(“973”计划 2009CB522902); 国家自然科学基金(30672720); 中国中医科学院自主选题(Z02011)
中文摘要:

目的:探讨热灸法的作用机制及最佳的热灸刺激参数。方法:选用SD大鼠15只,微电极胞外记录延髓背侧网状亚核(SRD)神经元活动,观察7种不同温度(40、42、44、46、48、50、52℃)和7种不同圆形面积(直径分别为1.0、1.5、2.0、2.5、3.0、3.5、4.0 cm)组成的不同组合的热刺激对SRD神经元放电活动的影响。刺激部位以"中脘"穴为中心,刺激持续时间为30 s。结果:40℃和42℃的热刺激在任何面积下对SRD神经元均无激活作用;44℃-Φ3.5 cm和44℃-Φ4.0 cm的刺激组合对SRD神经元有明显激活作用;温度达46℃以上后,热刺激面积与SRD神经元的放电活动呈线性相关;48℃-Φ3.0 cm、50℃-Φ3.5 cm、52℃-Φ3.5 cm刺激组合达到SRD神经元放电活动的平台期,在温度不变的条件下,SRD神经元放电不再随刺激面积的增大而增加。结论:伤害性范围内的热刺激可以激活SRD神经元,当热刺激达到一定参数后,SRD神经元的激活反应会出现平台期。

英文摘要:

Objective To observe the effect of regional thermal(moxibustion-like) stimulation on discharges of neurons in the medullary subnucleus reticularis dorsalis(SRD) and to study the best thermal stimulation parameters in the rat.Methods Experiments were performed on 15 male Sprague-Dawley rats under anesthesia(10% urethane,1.0-1.5 g/kg).Unit discharges of single neurons in the medullary SRD were recorded extracellularly with glass micropipettes.Thermal stimulation(warm water filled in a glass bottle) with different temperature(40 ℃,42 ℃,44 ℃,46 ℃,48 ℃,50 ℃,52 ℃) and covering different area(diameter: 1.0 cm,1.5 cm,2.0 cm,2.5 cm,3.0 cm,3.5 cm,4.0 cm) was applied to "Zhongwan"(CV 12) region for 30 s.Firing rates of SRD neurons were analyzed by using Power-Lab Chart 5.0.Results When thermal stimulation with temperature of 40 ℃ and 42 ℃ and the stimulated area of 1.0-4.0 cm in diameter was applied to CV 12 region,discharges of the medullary SRD neurons had no obvious changes.When the temperature was increased to 44 ℃and 46 ℃,the electrical activities of SRD neurons were increased linearly along with the increase of the stimulated area of 1.0-4.0 cm in diameter.When the temperature was increased further from 48 ℃ to 52 ℃,the increased electrical activities of SRD neurons peaked at the stimulated area of 3.5 cm in diameter.In addition,thermal stimulation at a temperature of 50 ℃ and an area of 4.0 cm in diameter induced a larger increase of discharges of SRD neurons in comparison with that of 46 ℃ plus an area of 3.5 cm/4.0 cm in diameter(P0.05).No significant differences were found between 50 ℃ and 52 ℃ at any stimulated areas mentioned above(P0.05).Conclusion Noxious thermal(44-52 ℃) stimulation of CV 12 region can activate SRD neuron,which reaches a plateau when the stimulated area is increased to a certain range.

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期刊信息
  • 《针刺研究》
  • 中国科技核心期刊
  • 主管单位:国家中医药管理局
  • 主办单位:中国中医科学院针灸研究所 中国针灸学会
  • 主编:朱兵
  • 地址:北京市东直门内南小街16号
  • 邮编:100700
  • 邮箱:zcyj2468@sina.com
  • 电话:010-84089344
  • 国际标准刊号:ISSN:1000-0607
  • 国内统一刊号:ISSN:11-2274/R
  • 邮发代号:82-171
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘(网络版),波兰哥白尼索引,荷兰文摘与引文数据库,美国生物医学检索系统,中国中国科技核心期刊,中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:11102