中文摘要：

针对氯仿熏蒸浸提法测定淹水土壤微生物生物量碳时存在的问题，进行了测定方法的研究，建立了适合淹水土壤微生物生物量碳测定的新方法：液氯熏蒸浸提-水浴法，即在淹水土壤加入一定量的液态氯仿后，直接置于常压下熏蒸，然后用0．5μmolL^-1K2SO4溶液浸提，随后将浸提液放置于100℃水浴中以排除其中的残余氯仿，最后采用TOC分析仪测定浸提液中的有机碳含量。本方法既符合氯仿熏蒸法的原理，在操作上又简便可行，是一种测定淹水土壤微生物生物量碳的理想方法。

英文摘要：

A chloroform-fumigation extraction--water bath method（CFEB） was developed for measuring microbial biomass C in waterlogged soils. Liquid chloroform was directly added to the waterlogged soil to be tested, which was fumigated for 24 h at 25 ℃ under normal atmospheric pressure and in darkness, and then were extracted with 0.5mol L^-1 K2SO4 solution. The extractions were carried out in 100℃ water bath for 45 ～ 60 rain to remove chloroform residue before measuring TOC. Non-fumigation controls were extracted directly with 0.5 mol L^-1 K2SO4 solution. The method was tested on seven paddy soils which were incubated aerobically or anaerobically and compared with the standard chloroform fumigation extraction （CFE）. The results of the tests were reliable and reproducible, suggesting that the CFEB is a rapid and effective method for measuring microbial biomass C in waterlogged soils.