中文摘要：

目的验证睾酮对成年C57BL／6近交系雌鼠的胰岛素敏感性的影响及其对脂肪组织胰岛素通路信号、炎症信号通路分子活性的影响。方法将8周龄C57BIM6雌性小鼠21只随机分为睾酮组（n=11，每天按1．0mg／100g体质量腹腔注射睾酮）及对照组（n=10，每天注射等体积sesame oil），共给药16周分别检测两组小鼠体质量和体脂含量的变化；分别于处理前（0周），处理后2、3和16周，行腹腔内注射葡萄糖耐量试验；于睾酮处理16周行腹腔胰岛素耐受试验（ITT）；两组小鼠均在治疗17周后被处死，ELISA检测两组血清白细胞介素（IL）-6、单核细胞趋化因子（MCP）-1的表达；免疫印迹检测两组脂肪组织胰岛素信号通路分子GSK-3B，InsR及NF-κBp65磷酸化的改变，巨噬细胞不同表型表面分子CD16／32、CD206的表达。结果（1）睾酮组与对照组成年雌鼠的体质量以及体脂含量差异无统计学意义（P〉0．05）；（2）处理2周睾酮组小鼠空腹血糖开始明显高于对照组（P〈0．05）；处理3周睾酮组IGTT曲线下面积开始明显大于对照组（P〈0．05），至16周后差异更显著（P〈0．01）；处理16周后睾酮组ITT曲线下面积极显著大于对照组（P〈0．01）；处理16周后睾酮组雌鼠血清及脂肪组织中的IL-6、MCP-1均较对照组明显（P〈0．05）；睾酮组脂肪组织的InsR、GSK．3B的磷酸化水平及CD206的表达均明显低于对照组（P〈0．05），而脂肪组织NF—κBp65磷酸化水平、CD16／32的表达明显高于对照组（P〈0．05）。结论睾酮不影响成年C57BL／6雌鼠的体质量及体脂含量，但是可以通过影响脂肪组织的胰岛素信号转导来诱导胰岛素抵抗的形成；通过活化炎症信号通路分子NF-κB促进促炎症因子生成，并促进巨噬细胞向M1型转化。

英文摘要：

Objectives Insulin resistance （IR） is a common manifestation in patients with polycystic ovarian syndrome （PCOS）. Both clinical observations and animal studies have demonstrated that IR could be induced by hyperandrogenemia, which is the charac-teristic of PCOS. Nevertheless, the mechanisms of IR in PCOS are still unclear especially at the molecular level. We conduct this study to ellucidate the effects of androgen on insulin sensitivity and chronic low-grade inflammation in adult C57BL/6 female mice. Methods Eleven adult female C57BL/6 mice aged 8 weeks weredaily injected with testosterone （1.0 mg/100 g body weight）which dissolved in sesame oil （experimental group T） for 16 weeks. Ten control mice were injected with sesame oil only （ group Con）. The changes of body weight and body fat content were detected. Intraperitoneal glucose tolerance tests （IGTT） were performed at 0, 2, 3 and 16 weeks treatment, blood from tail vein was taken to detect levels of glucose. Intraperitoneal insulin tolerance tests （ITT） were performed at 16 weeks treatment. Both groups were sacrificed afterl6 weeks treatment, and phosphorylation of GSK313and InsR, two molecules of insulin signaling pathway, were detected by Western blot from adipose tissue. The phosphorylation of NF-κBp65, CD16/32, and CD206 were also detected in adipose tissues. ELISA was used totest the serum IL-6 and MCP-1. Results （ 1 ） No obvious significance of body weight as well as body fat content was detected between both experimental groups （ P 〉 0.05 ） ; （ 2 ） 2 weeks treatment with testosterone induced the in-crease of fasting blood glucose and displayed obvious significance compared with group Con （ P 〈 0. 05 ） ; （ 3 ） 3 weeks treatment with testosterone induced the increase of area under the curve （AUC） of the blood glucose following IGTT and displayed significance compared with group Con （P 〈 0. 05 ）. And more obvious significance was detected at 16 weeks treat-ment （P 〈0.01 ）;（4） 16 week