中文摘要：

将小鼠5d龄ES-D3细胞源的类胚体（Eas）单细胞，按5×10^4／mL接种6孔细胞培养板，在DMEM基础培养基中使EBs单细胞贴壁培养。于第14-21天时，分别添加60％成骨细胞条件培养液（A组）、50μg／mL维生素c＋50mmol／L β-磷酸甘油（B组）和50μg／mL维生素C＋50mmol／L β-磷酸甘油＋1μmol／L地塞米松（C组），并设不添加诱导剂对照组（D组）。第22天时用1％茜素红染色显示阳性细胞，计算成骨细胞诱导形成率，数据用方差分析和多重比较检验。结果表明，A组细胞增殖呈团状聚集，茜素红染色阳性细胞结节多分布于聚集的细胞群内及其边缘，成骨细胞诱导形成率为10．04％，与对照组比较，差异极显著（P〈0．01）；B组诱导分化的细胞分泌物形成网状结构，茜素红染色阳性细胞分布在这些网状结构中，成骨诱导形成率为7．43％，与对照组比较差异显著（P〈0．05）；C组茜素红染色阳性细胞分散而均匀，但未出现网状结构，成骨细胞诱导形成率提高至27．57％，与对照组比较差异极显著（P〈0．01）。

英文摘要：

The cells of 5-day embryoid bodies （EBs） derived from routine ES-D3 lineage were inoculated into each well of 6-well culture plate at 5×10^4/mL in DMEM. During the period from 14 to 21 days of the EBs-derived single cells attachment DMEM was supplemented with the concentration of 60% of conditioned-medicum（group A）, 50 μg/mL ascorbic acid＋50 mmol/L β-glycerophos- phate（group B）, 50 μg/mL ascorbic acid-1-50 mmol/L β- glycerophosphate ＋ 1 μmol/L dexamethasone（group C）, and without inducer （group D）, respectively, and at the 22th day the positive cells were characterized by the formation of discrete mineralized bone nodules that were stained with 1% alizarin red S, and the ratio of obstoblast differentiation were calculated and analyzed by variance analysis and multiple comparison. The results indicated that in group A the cells were dump-like growth and the positve cells stained with alizarin red mostly located inside or around, and the ratio ofobstoblast differentiation was 10.04%, which was extremely significent differences with group D（P 〈0.01）. In group B the secretion of differentiated cells formed net-like structures, and the stained-positive cells located in the centre of the structures and the ratio of obsteoblast differentiation was 7 A3%（P 〈0.05）. In group C the differentiated obsteoblats spreaded uniformly without net-like structures, and the ratio of obsteoblast differentiation was 27.57% （P〈0.01）.