为研究牛膝多糖对血鹦鹉部分非特异性免疫与脂类代谢指标的影响,以初始体质量为(48.0±3.5)g的血鹦鹉Cichlasoma citrinellum♂×C.synspilum♀为试验对象,配制脂肪水平分别为8%、14%的基础饲料和高脂饲料,并在高脂饲料中添加质量分数分别为0(对照)、0.05%、0.10%、0.20%、0.40%的牛膝多糖作为饲料添加剂,试验共分为6个处理组(基础饲料组、对照组和A-D试验组),每个处理组设3个平行,每个平行放25尾血鹦鹉,用基础饲料及5种试验饲料分别投喂6组试验鱼,养殖周期为28 d,分别于试验开始后第7、14、21、28天取样测定并进行分析。结果表明:随着牛膝多糖添加量的增加,血鹦鹉肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、溶菌酶(LZM)活力显著提高(P〈0.05),丙二醛(MDA)含量显著降低(P〈0.05);随着牛膝多糖添加量的增加,血清中谷丙转氨酶(ALT)活力呈降低趋势,且在添加量为0.20%时与对照组有显著性差异(P〈0.05),而谷草转氨酶(AST)活力则无显著性变化(P〉0.05);随着牛膝多糖添加量的增加,血糖(Glu)含量显著降低(P〈0.05),而甘油三酯(TG)和总胆固醇(CHOL)含量变化不明显(P〉0.05),肝脏脂蛋白脂酶(LPL)和脂肪酸合成酶(FAS)活力也未出现显著变化(P〉0.05)。研究表明,饲料中使用牛膝多糖作为添加剂,可提高血鹦鹉肝脏抗氧化能力与LZM活力,同时起到降血糖的作用,建议牛膝多糖添加量为0.40%,并连续投喂14 d。
A feeding trial was conducted to investigate effects of dietary achyranthes bidentata polysaccharide(ABP) levels on partial non-specific immunity and lipid metabolism in blood parrot(Cichlasoma citrinellum♂×C.synspilum♀).Blood parrot with body weight of(48.0±3.5) g were fed apparent satiation diets containing fat at rate of 8% and 14% of the basal diet and the high-fat diet supplemented with achyranthes bidentata polysaccharide at a rate of 0,0.05%,0.10%,0.20% and 0.40% for 28 d with triplication to evaluate the feasible use of ABP in aquaculture.The indices of partial non-specific immunity and lipid metabolism were determined in the blood parrot fed the diets 7,4,21,and 28 days after administration.The results showed that,as increase in addition of ABP,activities of superoxide dismutase(SOD),catalase(CAT),and lysozyme(LZM) were increased and levels of malondialdehyde(MDA) was decreased significantly(P 0.05) without significant effect(P 0.05) on activities of lipoprotein lipase(LPL) and fatty acid synthase(FAS) in liver.In serum,however,alanine aminotransferase(ALT) activity and blood glucose(Glu) level were decreased significantly(P〉0.05) without significant effect(P〈0.05) on aspartate aminotransferase(AST) activity(except for the fish fed the diet containing 0.20% of ABP,and levels of triglyceride(TG),and total cholesterol(CHOL).In summary,dietary ABP) leads to improve antioxidant capacity and lysozyme activity in liver,and to reduce liver injury and has quite obvious fuctions in the diabetic treatment in blood parrot.It is recommended that 0.40% ABP be of the optimal dietary dose with 14 days continuous feeding.