中文摘要：

目的：在裸鼠体内实验中，观察使用人端粒酶逆转录酶（hTERT）核心启动子引导人钠碘转运体（hNIS）基因的重组腺病毒介导放射性核素显像的可行性。方法：扩增重组腺病毒，测定转染后U87细胞摄碘率，构建U87人胶质瘤荷瘤裸鼠模型，使用重组腺病毒Ad—hTERT—hNIS进行体内转染，进行放射性核素^99Tcm显像。结果：重组腺病毒Ad—hTERT—hNIS转染端粒酶阳性的U87细胞后，hNIS基因能够介导胶质瘤细胞摄碘，并且该病毒能对U87胶质瘤荷瘤裸鼠进行体内转染；成功介导靶向性hNIS基因的表达，而转染后的荷瘤裸鼠模型能成功介导放射性核素9STcmO2^-显像。结论：重组腺病毒Ad—hTERT—hNIS能够实现裸鼠体内hNIS基因的靶向性表达，并能介导放射性核素99Tcm显像。

英文摘要：

Objective： To investigate the possibility of transfecting human sodium iodide symporter （hNIS） gene following human telom- erase reverse transcriptase promoter （hTPO） into U87 malignant glioma nude mice for radioactive nuclide imaging in vivo. Methods： The recombinant adenovirus were amplified and purified, and the ^125I-iodide uptake of transfected U87 cells was detected. The malignant glioma nude mice animal model was performed, Ad-hTERT-hNIS was transfected in vivo, then radioactive nuclide ^99Tcm tumor imaging was obtained. Results： After U87 cells of telomerase-positive were transfected with Ad-hTERT-hNIS, Ad-hTERT-hNIS could mediate ^125I uptake of malignant glioma cell and malignant glioma nude mice animal model could be transfected by Ad-hTERT-hNIS, result in tumor specific hNIS gene expressioning and radioactive nuclide ^99Tcm tumor imaging. Conclusion： The recombinant adenovirus Ad- hTERT-hNIS could lead tumor specific hNIS gene expression in malignant glioma nude mice animal model and show ^99Tcm tumor imaging.