中文摘要：

目的研究大鼠局灶性大脑中动脉缺血再灌注过程中缺血脑区和远隔脑区氨基酸动态变化的差异。方法颈内动脉插线法制备大鼠大脑中动脉缺血再灌注模型,采用微透析技术取样,高效毛细管电泳-激光诱发荧光法检测,分别观察局灶性脑缺血再灌注过程中缺血脑区（纹状体）和远隔脑区（海马）细胞外3种氨基酸含量的动态变化。结果纹状体细胞外谷氨酸、甘氨酸及γ-氨基丁酸3种氨基酸含量在局灶性大脑中动脉缺血20min时即显著升高,再灌注后有所下降,在缺血60min及再灌注60min一直维持在显著高于基线值的水平;海马细胞外3种氨基酸含量缺血20~40min明显升高,以后有所回降,再灌注20-40min又上升,缺血期和再灌注期分别出现一次高峰;假手术组动物纹状体和海马细胞外3种氨基酸含量在相应各时间点与基线值相比无显著变化。结论局灶性大脑中动脉缺血再灌注不仅导致缺血脑区的损伤,在远隔脑区也引起相应的病理生理变化。

英文摘要：

Objective To explore the difference in dynamic changes of the extracellular amino acids concentrations in ischemia region and remote region during focal cerebral ischemia-reperfusion. Methods A rat model of middle cerebral artery occlusion was used to produce focal brain ischemia-reperfusion with ischemia core and remote region. In vivo brain microdialysis sampling and high performance capillary electrophoresis detecting were applied to monitor the dynamic changes in extracellular concentrations of amino acids in different encephalic regions. Results The extracellular concentrations of 3 amino acids, Glu Gly and GABA, in ischemia region （striatum） were increased at 20 min after middle cerebral artery occlusion, and maintained at high level in the 60 min of ischemia and following 60 min of reperfusion. The extracellular concentrations of the 3 amino acids in remote region （hippocampus） were also increased at 20 min after middle cerebral artery occlusion, and the dynamic change had two characteristic peaks during ischemia period and reperfusion period. The extracellular concentrations of the 3 amino acids in striatum and hippocampus in sham-operated group had no significant change at corresponding time points. Conclusion Focal brain ischemia-reperfusion not only caused damage in ischemia region, but also resulted in the corresponding physiopathologic changes in remote region.